| 沉默USP39对食管癌细胞增殖的影响 |
| 投稿时间：2016-04-17 修订日期：2016-05-02 点此下载全文 |
| 引用本文：顾怡雯,江伟骏,李锦伟.沉默USP39对食管癌细胞增殖的影响[J].医学研究杂志,2016,45(12):76-79,104 |
| DOI：
10.11969/j.issn.1673-548X.2016.12.021 |
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| 中文摘要:目的 探索食管癌细胞EC9706及人正常食管上皮细胞HEEC中USP39分子的表达差异及沉默USP39对食管癌细胞EC9706增殖的影响。方法 利用qRT-PCR及Western blot法检测细胞中USP39表达情况；设计并合成USP39的siRNA （USP39-siRNA）及对照（USP39-NC）转染食管癌EC9706细胞，利用qRT-PCR、Western blot法检测EC9706细胞中USP39的表达变化；MTT、平板克隆实验检测EC9706细胞的增殖。结果 USP39在EC9706细胞中的表达高于人正常食管上皮细胞HEEC的表达，USP39-siRNA下调了EC9706细胞中USP39基因水平与蛋白水平的表达，并抑制了细胞的增殖能力。结论 USP39-siRNA能够下调USP39的表达，并有效抑制食管癌EC9706细胞的增殖，为以USP39为靶点的食管癌基因治疗奠定基础。 |
| 中文关键词:USP39 EC9706细胞 增殖 食管癌 |
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| Effect of Silencing UAP39 Expression on the Proliferation of Esophagus Cancer Cells |
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| Abstract:Objective To observe the different expression of USP39 in esophagus cancer cell and normal esophageal epithelial cells, and study the effect on esophagus cancer cell proliferation by knocked down the expression of USP39.Methods siRNA fragments (USP39-siRNA)and USP39-NC were designed and synthesized according to gene sequences of USP39 and transfected to the esophagus cancer cells. The mRNA and protein expression levels of USP39 were detected using qRT-PCR and western blot respectively; cell proliferation was detected by MTT and colony formation assay in EC9706 cells.Results The mRNA and protein expression levels of USP39 was higher in EC9706 cells than in HEEC cells, and the expression level of USP39 was reduced by USP39-siRNA in the EC9706 cells, the proliferation of EC9706 cells decreased after transfected with USP39-siRNA.Conclusion USP39-siRNA could down-regulate the expression of USP39 and inhibit the proliferation of EC9706 cells, which is a theory foundation for USP39 as a gene target therapy of esophagus cancer. |
| keywords:USP39 EC9706 cells Proliferation Esophagus cancer |
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