| ACTN4对胃癌细胞侵袭转移的影响 |
| 投稿时间：2016-04-25 修订日期：2016-05-08 点此下载全文 |
| 引用本文：夷青,申俊龙,刘亚斌.ACTN4对胃癌细胞侵袭转移的影响[J].医学研究杂志,2016,45(12):93-96 |
| DOI：
10.11969/j.issn.1673-548X.2016.12.025 |
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| 中文摘要:目的 研究α-辅肌动蛋白（alpha-actinin-4，ACTN4）在胃癌SGC7901细胞及正常胃黏膜上皮RGM-1细胞中的表达及对胃癌SGC7901细胞侵袭和转移的影响。方法 通过qRT-PCR技术及蛋白印迹技术检测ACTN4在胃癌SGC7901细胞及RGM-1正常胃黏膜上皮细胞中的表达；利用RNAi技术敲低SGC7901细胞中的ACTN4表达，并应用qRT-PCR及蛋白印迹技术检测SGC7901细胞中ACTN4的沉默效果；通过Transwell实验检测细胞侵袭及转移能力的变化。结果 ACTN4在胃癌SGC7901细胞中明显高表达；ACTN4-siRNA可有效敲低SGC7901细胞中ACTN4的表达，敲低ACTN4的表达可以明显降低胃癌SGC7901细胞的侵袭转移能力。结论 ACTN4在胃癌细胞发生侵袭转移中发挥着重要作用。 |
| 中文关键词:α-辅肌动蛋白 SGC7901细胞 侵袭转移 胃癌 |
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| Effect of ACTN4 on Invasion and Migration of Gastric Cancer Cells |
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| Abstract:Objective To investigate the expression of ACTN4 in gastric cancer cells SGC7901 and normal gastric mucosa epithelial cells RGM-1 and detect the effect of ACTN4 on the invasion and migration of gastric cancer cells SGC7901.Methods The expression of ACTN4 in SGC7901 cells and RGM-1 cells was detected by qRT-PCR and western blotThe scilencing effect of ACTN4-siRNA was detected by qRT-PCR. After siRNA and control siRNA transfected into SGC7901 cells,the invasion and migration ability of SGC7901 cells were analyzed by transwell assay.Results The expression of ACTN4 in SGC7901 cells was higher than in RGM-1 cells. ACTN4 expression of SGC7901 cells was effectively silenced by ACTN4-siRNA and the invasion and migration abilities of SGC7901 cells decreased after transfected ACTN4-siRNA.Conclusion ACTN4 may play an important role in the invasion and migration of human gastric cancer. |
| keywords:Alpha-actinin-4(ACTN4) SGC7901 cells Invasion and migration Gastric cancer |
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