波形蛋白影响PC12细胞神经分化的研究
投稿时间:2016-05-29  修订日期:2016-05-30  点此下载全文
引用本文:邵晓婷,陈琪,郭俊霞,吴丽慧.波形蛋白影响PC12细胞神经分化的研究[J].医学研究杂志,2017,46(1):22-26
DOI: 10.11969/j.issn.1673-548X.2017.01.007
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作者单位E-mail
邵晓婷 325003 温州医科大学附属第二医院育英儿童医院  
陈琪 325003 温州医科大学附属第二医院育英儿童医院  
郭俊霞 325003 温州医科大学附属第二医院育英儿童医院  
吴丽慧 325003 温州医科大学附属第二医院育英儿童医院
310053 杭州医学院 
lhwu@wzmc.net 
基金项目:国家自然科学基金资助项目(81271505);国家卫生部浙江省联合重点科研项目(WKJ2012-2-018)
中文摘要:目的 探究波形蛋白分子在PC12细胞神经分化中的作用。方法 用免疫印迹方法检测PC12H和PC12L细胞中波形蛋白的表达情况。用免疫印迹方法检测PC12H细胞和PC12L细胞在NGF处理前后波形蛋白的表达情况。构建波形蛋白干扰质粒,用免疫印迹方法检测该质粒的干扰效果。分别将对照质粒、波形蛋白干扰质粒瞬时转染PC12H细胞,培养0、1、2、3天观察与记录细胞的分化情况,用Image-pro plus软件定量分析这两组细胞的平均神经突长度和含分化神经突细胞的百分比例。结果 PC12H细胞中波形蛋白的表达明显高于PC12L细胞(P=0.000)。NGF处理后PC12H和PC12L细胞中波形蛋白的表达量均增加,且在PC12L细胞中更明显(P=0.000)。波形蛋白干扰质粒能有效地抑制PC12H细胞中波形蛋白的表达(P=0.000)。瞬时转染波形蛋白干扰质粒能有效地抑制PC12H细胞的神经分化,定量分析结果显示干扰组细胞的平均神经突长度和含分化神经突细胞的百分比均明显小于对照组,差异均有统计学意义(P=0.000)。结论 波形蛋白分子可以促进PC12细胞的神经分化。
中文关键词:波形蛋白  PC12细胞  神经分化
 
Effect of Vimentin on Neural Differentiation of PC12 Cells
Abstract:Objective To explore the function of vimentin on neural differentiation of PC12 cells. Methods Western blot was used to detect vimentin expression between PC12H and PC12L cells. Western blot was applied to detect vimentin expression of PC12 cells before and after NGF treatment. Vimentin interference vector was constructed and the interference effect of vimentin expression was tested by Western blot. PC12H cells were transiently transfected with control vector, vimentin interference vector, respectively. After cultured for 0day, 1day, 2days, 3days, we observed and photographed the morphology of cells. The neurite length and the population of neurite-branching cells were quantified by Image-pro plus software. Results Vimentin expression in PC12H cells was higher than PC12L cells (P=0.000). Vimentin expression in PC12 cells was increased after NGF treatment and the change was more remarkable in PC12L cells (P=0.000). Vimentin interference vector had a noteworthy suppression effect on vimentin expression (P=0.000). PC12H cells transiently transfected with vimentin interference vector had lower differentiation morphologies compared to control ones. The neurite length of cells was shorter and the population of neurite-branching cells was lower in interference vector group than control one, difference was statistically significant (P=0.000). Conclusion Vimentin can promote nueral differentiation of PC12 cells.
keywords:Vimentin  PC12 cells  Neural differentiation
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