| 压力负荷诱导的大鼠舒张性心力衰竭模型的改良及效果探讨 |
| 投稿时间：2016-04-30 修订日期：2016-05-25 点此下载全文 |
| 引用本文：刘静,崔海峰,李思耐,林谦.压力负荷诱导的大鼠舒张性心力衰竭模型的改良及效果探讨[J].医学研究杂志,2017,46(1):34-38,52 |
| DOI：
10.11969/j.issn.1673-548X.2017.01.010 |
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| 基金项目:国家自然科学基金资助项目（面上项目）（81373779） |
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| 中文摘要:目的 利用改良术式制备压力负荷诱导大鼠舒张性心力衰竭模型并探讨其效果。方法 Wistar大鼠40只随机分为正常组、腹主动脉缩窄0.6、0.7及0.8mm组。3组模型大鼠利用改良术式将腹主动脉分别缩窄至0.6、0.7、0.8mm，观测术后大鼠存活率。术后8周及12周进行超声心动检测，术后12周检测血流动力学舒张功能指标。结果 术后模型组存活率分别为：缩窄0.6mm组46.7%，缩窄0.7mm组66.7%，缩窄0.8mm组80%。超声心动图：与对照组比较，缩窄0.6mm组：术后8周LVPWd（2.34±0.51mm）、LVmass（1.42±0.20g）、E/e（11.62±8.45）显著增加（P<0.05）；术后12周LVPWd（2.47±0.50mm）、LVmass（1.75±0.31g）、E/e（19.67±4.28）显著增加，IVRT（19.68±4.05ms）显著缩短（P<0.05），其他指标差异无统计学意义（P>0.05）；且E/e组内比较差异有统计学意义（P<0.05）；缩窄0.7mm组：术后8周，仅LVIDd（8.55±0.56mm）显著增加（P<0.05），其余指标差异无统计学意义（P<0.05）；术后12周，LVPWd（2.16±0.37mm）、LVmass（1.44±0.16g）、E/e（10.51±5.32）显著增加（P<0.05），其他指标差异无统计学意义（P<0.05）；且E/e组内比较差异有统计学意义（P<0.05）。缩窄0.8mm组：术后第8周及12周各指标差异均无统计学意义（P>0.05）。血流动力学：模型3组术后12周LVEDP（23.66±10.41、16.75±4.68、14.83±5.92mmHg）均较正常组显著升高（P<0.05）；缩窄0.6mm组及缩窄0.7mm组Tau值（11.32±3.33、9.01±2.74ms）较正常组显著延长（P<0.05）；缩窄0.6mm组-dp/dt值（-36343.30±991.95）较正常组显著降低（P<0.05）。结论 缩窄0.6mm组术后第8周出现舒张性功能不全，术后第12周出现舒张性心力衰竭，成模速度快且模型稳定，但存活率低；缩窄0.7mm术后12周才出现舒张性功能障碍，成模速度较慢但存活率相对较高。改良术式操作简便，可降低手术危险性，具有较广阔的应用前景，更适合于舒张性功能不全模型的制备。 |
| 中文关键词:舒张性心力衰竭 腹主动脉缩窄 动物实验 模型制备 |
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| Observation on the Validity of Modified Operation for Pressure Load Induced Heart Failure with Preserved Ejection Fraction Model Establishment in Rats |
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| Abstract:Objective To explore the validity of modified operation for pressure load induced heart failure with preserved ejection fraction (HFPEF) model establishment in rats. Methods Forty Wistar rats were divided into no intervention, constricting 0.6mm, 0.7mm and 0.8mm of abdominal aorta groups randomly. We observed death rate of rats in these groups at the point of 4 weeks after operation, and compared the parameters of echocardiography between these groups at the points of 8, 12 weeks after operation, and of hemodynamic at the point of 12 weeks after operation. Results Death rate:46.7% of constricting 0.6mm group, 66.7% of constricting 0.7mm group and 80% of constricting 0.8mm group. Parameters of echocardiography:When compared with no intervention group, LVPWd (2.34±0.51mm), LVmass (1.42±0.20g) and E/e (11.62±8.45) of constricting 0.6mm group increased statistically significantly (P<0.05).at the point of 8 weeks after operation; moreover, LVPWd (2.47±0.50mm), LVmass (1.75±0.31g) and E/e (19.67±4.28) increased statistically significantly (P<0.05) at the point of 12 weeks after operation, however IVRT (19.68±4.05ms) decreased statistically significantly (P<0.05); when compared within groups, only E/e showed statistical significance at the point of 12 weeks after operation (P<0.05).When compared with no intervention group, only LVIDd (8.55±0.56mm) of constricting 0.7mm group increased statistically significantly (P<0.05).at the point of 8 weeks after operation, however LVPWd (2.16±0.37mm), LVmass (1.44±0.16g) and E/e (10.51±5.32) increased significantly (P<0.05).at the point of 12 weeks after operation; when compared within groups, only E/e showed statistical significance (P<0.05).at the point of 12 weeks after operation. No significant difference was detected in all parameters in constricting 0.8mm group no matter at the point of 8 or 12 weeks after operation (P>0.05). Parameters of hemodynamic:when compared with no intervention group, all groups showed statistical difference in LVEDP (23.66±10.41, 16.75±4.68, 14.83±5.92mmHg) (P<0.05); for Tau value, statistical differences were only detected in groups of constricting 0.6mm and 0.7mm (11.32±3.33, 9.01±2.74ms), (P<0.05), but for -dp/dt, only constricting 0.6mm group showed statistical difference (-36343.30±991.95), (P<0.05). Conclusion The rats of constricting 0.6mm group showed signs of diastolic dysfunction at the point of 8 weeks after operation, and signs of HFPEF at the point of 12 weeks after operation, which HFPEF model establishment was faster and more stable but with a higher death rate. Although with a lower death rate, the HFPEF model establishment was barely satisfactory in constricting 0.7mm group. The modified operation for pressure load induced HFPEF model establishment is easy to handle and bears lower surgical risk, which will have a wider prospect and be more suitable for the preparation of diastolic dysfunction model. |
| keywords:HFPEF Constriction of abdominal aorta Animal experiment Model preparation |
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