| 不同浓度过氧化氢对人脐静脉内皮细胞增殖活性的影响 |
| 投稿时间：2016-05-05 修订日期：2016-05-07 点此下载全文 |
| 引用本文：吴芳园,廖海涛,韦义萍.不同浓度过氧化氢对人脐静脉内皮细胞增殖活性的影响[J].医学研究杂志,2017,46(1):46-48 |
| DOI：
10.11969/j.issn.1673-548X.2017.01.013 |
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| 基金项目:国家自然科学基金资助项目（81260290） |
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| 中文摘要:目的 建立氧化应激损伤模型，研究过氧化氢（H202）对内皮细胞增殖活性的影响，为深入探讨氧化应激致血管内皮细胞损伤机制奠定基础。方法 以0、50、100、150、300、400和600μmol/L H202作用于人脐静脉内皮细胞（HUVEC），在8、12和24h时间段内采用MTT法测定细胞的增殖活性。结果 50μmol/L H2O2作用8h对HUVEC活性无影响，与对照组比较，差异无统计学意义（P>0.05）；其余各浓度各时间段内，H2O2均可引起细胞活性降低，与对照组比较，差异有统计学意义（P<0.01）。各两组比较，8h组与12h组、24h组比较，差异有统计学意义（P<0.05）；12h组与24h组比较，差异无统计学意义（P>0.05）。12h的IC50为133.3μmol/L。结论 在一定浓度时间范围内，H202对HUVEC有增殖抑制作用，150μmol/L H2O2处理12h为最佳实验浓度时间。 |
| 中文关键词:过氧化氢 浓度 内皮细胞 增殖 |
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| Effect of Different Concentrations of Hydrogen Peroxide on Proliferation of Human Umbilical Endothelial Cells |
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| Abstract:Objective To establish oxidative stress injury model and investigate the effect of hydrogen peroxide on proliferation of endothelial cells, in order to lay a foundation for further research on the mechanism of oxidative stress induced damage to vascular endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were induced by 0,50,100,150,300,400 and 600μmol/L H2O2, and MTT assay was use to determine the proliferation of endothelial cells in 8h,12h and 24h. Results There was no effect on HUVEC cells activity after induced by 50μmol/L H2O2 for 8h, compared to the control group, and the difference was not statistically significant (P>0.05). H2O2 could cause HUVEC cells activity decreased with other concentration of each time, compared to the control group,and the difference was great statistically significant (P<0.01). In multiple comparisons, the difference between 8h group and 12h group,24h group was statistically significant (P<0.05); the difference between 12h group and 24h group was not statistically significant (P>0.05). The IC50 of 12h was 133.3μmol/L. Conclusion In a certain time and concentration range, H2O2 had proliferative effect on HUVEC cells. The best experimental H2O2 concentration and time were 150μmol/L and 12h. |
| keywords:Hydrogen peroxide Concentrations Endothelial cells Proliferation |
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