| 硼替佐米对人脐动脉血管平滑肌细胞NF-κB的影响 |
| 投稿时间：2016-05-11 修订日期：2016-05-23 点此下载全文 |
| 引用本文：魏会丽,王莹,孙瑞红.硼替佐米对人脐动脉血管平滑肌细胞NF-κB的影响[J].医学研究杂志,2017,46(1):62-66 |
| DOI：
10.11969/j.issn.1673-548X.2017.01.018 |
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| 基金项目:黑龙江省自然科学基金资助项目（H201306）；黑龙江省科研计划项目（201602） |
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| 中文摘要:目的 研究不同浓度硼替佐米对人脐动脉血管平滑肌细胞（vascular smooth muscle cell，VSMC） NF-κB表达及活性的影响。方法 实验分组：1对照组：常规培养的血管平滑肌细胞；24nmol/L硼替佐米组；310nmol/L硼替佐米组；440nmol/L硼替佐米。以贴壁法培养人脐动脉VSMC，将培养的4~6代细胞种于培养皿中，在培养液中加入上述不同浓度的硼替佐米处理48h，并确定VSMC的最佳状态，用免疫印记（Western blot）法检测细胞中NF-κB总蛋白表达水平；采用EMSA法检测细胞核中NF-κB的活性。结果 对照组、4nmol/L硼替佐米组、10nmol/L硼替佐米组和40nmol/L硼替佐米组的血管平滑肌细胞中NF-κB蛋白水平分别为0.554±0.012，0.475±0.030，0.377±0.044，0.216±0.026，与对照组相比，4nmol/L硼替佐米组的NF-κB蛋白表达水平降低（P<0.05），而10nmol/L硼替佐米组和40nmol/L硼替佐米组的NF-κB蛋白表达水平均较对照组明显降低（P<0.01）。EMSA法检测的结果显示随着硼替佐米浓度的增加，NF-κB在核内的活性呈下降趋势。结论 硼替佐米能够抑制NF-κB蛋白的表达及活性，且呈剂量依赖性。 |
| 中文关键词:泛素蛋白酶体系统 硼替佐米 血管平滑肌细胞 NF-κB 炎性反应 |
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| Effects of Bortezomib on NF-κB of Human Umbilical Artery Vascular Smooth Muscle Cells |
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| Abstract:Objective To study the effects of different concentrations of ubiquitin proteasome inhibitor bortezomib on NF kappa B expression and activity of human umbilical artery vascular smooth muscle cells. Methods Experimental groups were as following: (1) control group:conventional cultured vascular smooth muscle cells; (2) 4nmol/L bortezomib group; (3) 10nmol/L bortezomib group; (4) 40nmol/L bortezomib. Adherent cultured human umbilical artery VSMC and cultured 4-6th generation cells were seeded in culture dishes. Cells were treated with different concentrations of bortezomib for 48 hours to determine the best state of VSMC. We used immunoblot (western blot) to detect in cells the total protein levels of NF-κB and EMSA to analyze nucleus NF-κB activity. Results In the control group, 4 nmol/L bortezomib group, 10nmol/L bortezomib group and 40nmol/L bortezomib group of vascular smooth muscle cells NF-κB protein levels were 0.554±0.012, 0.475±0.030, 0.377±0.044 and 0.216±0.026, respectively. Compared with the control group, the NF-κB protein levels in the 4nmol/L bortezomib group decreased (P<0.05), while in both 10nmol/L bortezomib group and 40nmol/L bortezomib group, the NF-κB protein levels were significantly lower than in the control group (P<0.01). EMSA assay results showed that with the increase of the concentration of bortezomib, NF-κB in the nucleus activity gradually decreased. Conclusion Bortezomib can inhibit the expression and activity of NF-κB protein in a dose-dependent manner. |
| keywords:Ubiquitin-proteasome system Bortezomib Vascular smooth muscle cells NF-κB Inflammation |
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