| 活动期肺结核外周血mir-144的表达水平及意义 |
| 投稿时间：2016-02-06 修订日期：2016-03-21 点此下载全文 |
| 引用本文：张立营,陈朴,高鹏,沈伟.活动期肺结核外周血mir-144的表达水平及意义[J].医学研究杂志,2017,46(1):151-154 |
| DOI：
10.11969/j.issn.1673-548X.2017.01.040 |
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| 中文摘要:目的 探讨活动期肺结核患者外周血mir-144表达水平及临床意义。方法 选择2014年1月~2015年1月在笔者医院就诊的20例活动期肺结核患者及20例健康体检者作为研究对象，收集其静脉血，分别提取两组患者外周血单个核细胞中总RNA，采用实时荧光定量PCR比较其外周血mir-144的表达水平情况，采用受试者工作特征曲线（receiver operating characteristic curve，ROC）评估mir-144的诊断效能。同时采用ELISA法测定两组研究对象血浆中的IL-6、TNF-α、IFN-γ的水平。进一步进行相关性分析评估外周血mir-144表达水平与IL-6、TNF-α、IFN-γ的相关性。结果 活动期肺结核患者外周血中mir-144表达水平相比较健康对照组高，差异有统计学意义（P>0.05）。活动期肺结核患者外周血中IL-6、TNF-α、IFN-γ的水平比健康对照组要高，差异有统计学意义（P<0.05）。ROC下面积为0.885，当最佳筛查阳性界值（cut-off值）为0.955时，敏感度和特异性均为85%。活动期肺结核外周血中mir-144的表达水平与其血浆中的IL-6、TNF-α、IFN-γ的水平呈负相关，相关系数分别为-0.905、-0.910、-0.892。结论 外周血单个核细胞中mir-144的表达水平可能作为活动期肺结核诊断的一个血清学指标，mir-144在结核杆菌感染后免疫反应中可能与外周血IL-6、TNF-α、IFN-γ的表达水平有关。 |
| 中文关键词:结核 外周血 Mir-144 细胞因子 |
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| Expression Level and Clinical Significance of mir-144 in Peripheral Blood of Active Pulmonary Tuberculosis Patients |
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| Abstract:Objective To evaluate the diagnostic values of microRNA-144 in peripheral blood of active pulmonary tuberculosis patients. Methods Twenty active pulmonary tuberculosis patients were selected between Jan 2014 and Jan 2015,and twenty healthy people, selected in parallel, served as the control group. Real-time PCR was used to detect mir-144 expression in PBMCs. Receiver operating characteristic curve (ROC) was employed to evaluate the diagnostic value of mir-144 in active pulmonary tuberculosis. Serum levels of IL-6, TNF-α and IFN-γ was detected by ELISA. Pearson correlation coefficient was used to evaluate the correlation between mir-144 expression and serum levels of IL-6, TNF-α and IFN-γ. Results The expression of mir-144 in active pulmonary tuberculosis patients was signifiantly higher than that in normal people. Serum levels of IL-6, TNF-α and IFN-γ were significantly higher in pediatric TB patients compared with normal people. The area under the curve (AUC) was 0.855. The cut-off threshold of mir-144 for active pulmonary tuberculosis diagnosis was 0.955 with a sensitivity of 85% and a specificity of 85%. Furthermore, mir-144 expression was negatively correlated with serum levels of IL-6 (r=-0.905), TNF-α (r=-0.910), and IFN-γ (r=-0.892). Conclusion mir-144 has the potential to be a diagnostic marker in active pulmonary tuberculosis patients. Moreover,the role of mir-144 in the immune response of mycobacterium tuberculosis infection may be related with serum levels of IL-6, TNF-α, IFN-γ. |
| keywords:Tuberculosis Peripheral blood Mir-144 Cytokines |
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