| 不同产地神曲甲醇提取物对巨噬细胞及小肠上皮细胞活性的影响 |
| 投稿时间：2016-02-18 修订日期：2016-03-24 点此下载全文 |
| 引用本文：刘晓瑜,高慧,刘腾飞,贾坤静,陈江宁,单国顺,张凡,贾天柱.不同产地神曲甲醇提取物对巨噬细胞及小肠上皮细胞活性的影响[J].医学研究杂志,2017,46(2):51-55 |
| DOI：
10.11969/j.issn.1673-548X.2017.02.015 |
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| 基金项目:国家科技重大专项基金资助项目（20102X09401-304-105A） |
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| 中文摘要:目的 比较不同产地神曲对小鼠RAW264.7单核-吞噬细胞及大鼠小肠上皮细胞株IEC-6细胞活性的影响。方法 不同产地神曲样品甲醇提取，氮吹仪下挥干溶剂，加入空白培养基配成1g/ml母液，无菌条件下加DMEM空白培养基使母液稀释成浓度分别为800、400、200、100、0μg/ml，对大鼠小肠上皮细胞株IEC-6细胞及小鼠RAW264.7单核-吞噬细胞进行给药培养，检测二者增殖率、巨噬细胞吞噬能力及其所分泌TNF-α的水平。结果 不同神曲样品在低给药浓度（100、200μg/ml）时均有促进IEC-6细胞及RAW264.7细胞增殖的作用，尤其以中国药材公司采集的神曲炒品（Z炒神曲）效果较显著（P<0.05）。对于巨噬细胞吞噬能力及其所分泌的TNF-α水平，各组间虽差异无统计学意义，但仍以Z炒神曲在较低浓度时优于其他组别。结论 不同产地神曲对小肠上皮IEC-6细胞及巨噬细胞RAW264.7活性有促进作用，以从中国中药公司采集的Z炒品效果较优。 |
| 中文关键词:中药 神曲 巨噬细胞 小肠上皮细胞 |
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| Effects of Methanol Extracts of Different Habitat Massa Medicata Fermentata(MMF) on the Activity of Macrophages and Small Intestinal Epithelial Cells |
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| Abstract:Objective To compare the effect of different habitat Massa Medicata Fermentata on the activity of the mouse mononuclear macrophages RAW264.7 and rat small intestinal epithelial IEC-6 cells. Methods Different MMF samples wera extracted with methanol drying solvent with nitrogen blowing instrument,1g/ml initial liquid was made with blank culture medium in a aseptic condition,the initial liquid was diluted into 800,400,200,100,0μg/ml with DMEM blank culture medium to culture mouse mononuclear macrophages RAW264.7 and rat small intestinal epithelial IEC-6 cells or determine the both appreciation rate,macrophage phagocytic ability and the levels of TNF-α. Results Different MMF samples had the effect of promoting the proliferation of IEC-6 cells and RAW264.7 cells at low concentration(100,200μg/ml,which especially remarkable was fried product collected from Chinese traditional Chinese medicine company(Z fried MMF)(P<0.05).Although there was no significant difference between the groups for the phagocytic ability of macrophages and its levels of TNF-α, Z fried product was better than other groups at low concentrations. Conclusion Different MMF samples have a promoting effect on the activity of intestinal epithelial IEC-6 cells and macrophage RAW264.7 cells.Z fried product collected from Chinese traditional Chinese medicine company is better. |
| keywords:TCM Massa Medicata Fermentata Macrophages Small intestinal epithelial cell |
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