microRNA-30b在非小细胞肺癌中的表达及预后分析
投稿时间:2016-05-31  修订日期:2016-06-27  点此下载全文
引用本文:齐泽铖,朱成楚,张波,陈保富,朱敏.microRNA-30b在非小细胞肺癌中的表达及预后分析[J].医学研究杂志,2017,46(2):63-65,82
DOI: 10.11969/j.issn.1673-548X.2017.02.018
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作者单位E-mail
齐泽铖 317000 温州医科大学附属台州医院心胸外科  
朱成楚 317000 温州医科大学附属台州医院心胸外科 zhucc669266@163.com 
张波 317000 温州医科大学附属台州医院心胸外科  
陈保富 317000 温州医科大学附属台州医院心胸外科  
朱敏 317000 温州医科大学附属台州医院心胸外科  
基金项目:浙江省医药卫生科技计划项目(2015KYA239)
中文摘要:目的 探讨微小RNA-30b(miRNA-30b)在非小细胞肺癌(NSCLC)中的表达情况,并进一步分析miR-30b与NSCLC患者预后的相关性。方法 收集41例NSCLC患者的肿瘤组织及其正常组织(距离肿瘤组织>5cm),采用real-time PCR法检测miR-30b的表达情况,并对预后进行分析。结果 肿瘤组织miR-30b的表达水平显著低于正常组织(P<0.01);miR-30b的表达水平与临床分期、淋巴结转移及EGFR基因突变显著相关(P<0.05)。Kaplan-Meier分析显示肿瘤组织中miR-30b高表达组的生存时间明显高于低表达组(P<0.05)。结论 MiR-30b在NSCLC中的表达是下调的,可作为NSCLC预后的一个新的潜在标志物。
中文关键词:miR-30b  非小细胞肺癌  EGFR  预后
 
Expression of MicroRNA-30b in Non-small Cell Lung Cancer and Its Prognostic Analysis
Abstract:Objective To investigate the expression of microRNA-30b(miRNA-30b) in patients of non-small cell lung cancer(NSCLC) and to analyse the association of its expression and prognosis. Methods The expression of miR-30b in 41 tumor tissues and paired normal tissues of NSCLC were detected by RT-PCR.The relationship between the expression levels of miR-30b and the prognosis of patients was analysed. Results The expression level of miR-30b was up-regulated in tumor tissues while compared with paired normal tissues(P<0.05).The expression of miR-30b was associated with TNM stages,lymphatic metastasis and the genetic mutation of EGFR(P<0.05).Kaplan-Meier analyse reveled that the overall survival of high-expression group was longer than the low-expression group(P<0.05). Conclusion The expression of miR-30b in NSCLC is down-regulated,and it is maybe a novle evaluation of predicting the prognosis of NSCLC.
keywords:miR-30b  NSCLC  EGFR  Prognosis
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