| Toll样受体4及下游炎性介质的检测在医院获得性肺炎中的临床应用 |
| 投稿时间：2016-05-17 修订日期：2016-07-01 点此下载全文 |
| 引用本文：罗建华,李俏俏,李隆祥,邱继海.Toll样受体4及下游炎性介质的检测在医院获得性肺炎中的临床应用[J].医学研究杂志,2017,46(2):138-141 |
| DOI：
10.11969/j.issn.1673-548X.2017.02.036 |
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| 基金项目:台州市淑江区科技计划项目（132057） |
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| 中文摘要:目的 分析Toll样受体4（TRL4）及下游炎性介质的检测在医院获得性肺炎（hospital acquired pneumonia，HAP）中的临床价值。方法 30例HAP患者按照数字表法随机分为干预组和实验组，另选取15例健康体检者作为对照组。3组患者均采集静脉血，其中干预组血样中加入TRL4 mAb 20μg，测定各患者外周血炎性因子水平，并提取外周血核细胞中总RNA，检测TLR4 mRNA的表达，同时进行基因测序，分析确定基因型。结果 干预组和实验组各炎性因子水平组间比较差异无统计学意义，但均显著高于对照组，差异具有统计学意义（P<0.05）。3组患者阳性表达率和相对表达量差异有统计学意义（P<0.05）。干预组阳性表达率和相对表达量均明显高于实验组和对照组，实验组也明显高于对照组，组间比较差异有统计学意义（P<0.05）。TLR-4阳性率与TNF-α、IL-1、IL-6、IL-10等下游炎性因子浓度水平均呈正相关，决定系数分别为0.732、0.714、0.695、0.753，具有显著相关性（P=0.000）。结论 医院获得性肺炎患者外周血中TRL4与下游炎性介质水平具呈正相关，且TRL4阳性率和炎性介质水平均高于健康人群，对医院获得性肺炎发病机制的进一步研究具有重要意义。 |
| 中文关键词:Toll样受体4 炎性介质 医院获得性肺炎 |
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| Toll-like Receptor 4 and Downstream Mediators of Inflammation Detection in the Clinical Application of Hospital Acquired Pneumonia |
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| Abstract:Objective To analyze toll-like receptor 4(TRL4) and downstream mediators of inflammation detection in hospital acquired pneumonia(hospital acquired root, HAP) of clinical value. Methods Thirty patients with HAP were divided into intervention group and experimental group according to the random number method.The other 15 cases were selected physical examination as a control. Three groups of patients were collected venous blood, of which the intervention group add 20 μg TRL4 mAb. Blood samples were wed to determine the peripheral blood in patients with inflammatory factor levels, and total RNA of the nuclear cells in peripheral blood, was extralted to detect the expression of TLR4 mRNA, simultaneously gene sequencing and determine the genotype. Results The various inflammatory factors levels had no significant difference between groups of, but they were significantly higher than the control group,and the difference was statistically significant,(P<0.05).Patients of the three groups with positive expression rate and relative expression had significant differences(P<0.05). Positive expression rate and the relative expression of intervention group were significantly higher than those of the experimental group and the control group, and experimental group was significantly higher than the control group(P<0.05).Statistics showed that TLR-4 positive rate and TNF alpha, IL-1, IL-6 and IL-10 downstream inflammatory factor levels all had positive correlation.Their determination coefficient were 0.732, 0.714, 0.695, 0.753(P=0.000). Conclusion Patients with hospital acquired pneumonia in the peripheral blood TRL4 and downstream level had a significant positive correlation. Inflammatory mediators and TRL4 positive rate and level of inflammatory medium were higher than healthy people. The hospital acquired pneumonia in the clinical diagnosis and condition prediction is of great significance. |
| keywords:Toll-like receptor 4 Inflammatory mediators Hospital-acquired pneumonia |
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