心肌线粒体改变对1型糖尿病小鼠心脏结构与功能的影响
投稿时间:2016-09-03  修订日期:2016-09-19  点此下载全文
引用本文:陈翠,孟哲颖,郑元义,胡兵,申锷.心肌线粒体改变对1型糖尿病小鼠心脏结构与功能的影响[J].医学研究杂志,2017,46(3):21-26
DOI: 10.11969/j.issn.1673-548X.2017.03.007
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作者单位E-mail
陈翠 200233 上海, 上海交通大学附属第六人民医院超声医学科  
孟哲颖 200233 上海, 上海交通大学附属第六人民医院超声医学科  
郑元义 200233 上海, 上海交通大学附属第六人民医院超声医学科  
胡兵 200233 上海, 上海交通大学附属第六人民医院超声医学科
200233 上海, 上海超声医学研究所 
 
申锷 200233 上海, 上海超声医学研究所
200336 上海, 上海交通大学附属同仁医院 
shene126@126.com 
基金项目:国家自然科学基金资助项目(面上项目)(81270208)
中文摘要:目的 探讨心肌线粒体改变对1型糖尿病小鼠心脏结构与功能的影响。方法 20只8周龄雄性C57/BL6J小鼠随机分为对照组(n=10),1型糖尿病组(n=10)。单次腹腔注射STZ(150mg/kg)建立1型糖尿病动物模型,实验到达终点时采用小动物用高分辨超声仪观察小鼠心脏结构,测量心脏功能;光镜下观察心肌细胞形态改变,透射电镜下观察心肌细胞线粒体变化,Western blot法检测线粒体相关蛋白TFAM及PGC-1α。原代培养C57BL/6J乳鼠心肌细胞,观察在不同糖浓度(5mmol/L、33mmol/L)中培养48h后心肌细胞的形态学变化,透射电镜下观察心肌细胞线粒体的改变,Western blot法检测线粒体相关蛋白TFAM及PGC-1α。结果 与正常组小鼠相比,1型糖尿病组小鼠心肌线粒体排列不规则,肿胀,脊脱落溶解,线粒体相关蛋白TFAM及PGC-1α表达减低(P< 0.05),1型糖尿病小鼠心脏肥大,室壁增厚,心功能减低。与正常糖浓度培养组相比,高糖培养组线粒体肿胀、脊断裂溶解,线粒体相关蛋白TFAM及PGC-1α表达减低(P<0.05),心肌细胞肥大显著,形态欠规则。结论 心肌线粒体结构与功能的改变与1型糖尿病小鼠心脏结构与功能改变有关。
中文关键词:1型糖尿病  心脏功能  心脏结构  心肌线粒体
 
Myocardial Mitochondria on Heart Structure and Function of Type I Diabetic Mice
Abstract:Objective To investigate the effect of mitochondrial changes on cardiac structure and function in type 1 diabetic mice. Methods ①Mice experiment:twenty 8 weeks old female C57/BL6J mice were randomly divided into two groups. Ten mice received STZ treatment to induce type 1 diabetes, and the other ten untreated mice served as control group. Cardiac function was analyzed by echocardiography, Cardiomyocyte morphological changes were observed by HE staining. Mitochondrial changes were examined by transmission electron microscope. The expression of TFAM, PGC-1α of myocardium were detected by Western blot. ②Cell experiment:neonatal mice cardiomyocytes were cultured with glucose in different concentrations (5mmol/L, 33mmol/L) for 48h. Cardiomyocyte morphological changes were observed by HE staining. Mitochondrial changes were examined by transmission electron microscope. The expression of TFAM, PGC-1α of myocardium were detected by Western blot. Results ①Cardiac function of Type I DM mice decreased while LVHW/BW,IVSD,LVPWD increased. Mitochondria swelled with raptured cristae. Expression of TFAM, PGC-1α of myocardium decreased. ②High glucose cultured cardiomyocytes showed hypertrophy and swollen mitochondria with raptured cristae, expression of TFAM, PGC-1α of myocardium decreased. Conclusion Changes of Myocardial mitochondria relate to heart structure and function of type I diabetic mice.
keywords:Type I diabetes  Cardiac function  Cardiac structure  Mitochondria
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