| 苯吸入对骨髓细胞凋亡和组蛋白去乙酰化酶影响 |
| 投稿时间：2016-07-18 修订日期：2016-08-02 点此下载全文 |
| 引用本文：葛杭萍,俞康,陈枫煜,施益芬,洪莉莉.苯吸入对骨髓细胞凋亡和组蛋白去乙酰化酶影响[J].医学研究杂志,2017,46(3):31-35 |
| DOI：
10.11969/j.issn.1673-548X.2017.03.009 |
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| 基金项目:国家自然科学基金资助项目（81172613，81502793） |
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| 中文摘要:目的 观察苯吸入对小鼠骨髓细胞损伤情况以及去乙酰化酶水平的变化。方法 自制动式苯吸入装置中放8~9周龄CD1雄性小鼠实验组吸入苯，正常对照组吸空气，6h/d，5天/周，300ml/m3及900ml/m3维持12周。末次吸苯后第2天，获取小鼠骨髓单个核细胞，利用流式细胞仪测骨髓细胞凋亡情况及用去乙酰化酶（HDAC）试剂盒测去乙酰化酶（HDAC酶）活性变化。结果 300ml/m3∶实验组小鼠骨髓细胞中早期凋亡细胞（AnnexinⅤ+PI-）比例（13.80%±5.31%）是对照组（8.33%±0.61%）的1.65倍（P<0.05）；晚期骨髓凋亡细胞（AnnexinⅤ+PI+）比例与对照组比较，差异无统计学意义（P>0.05）。900ml/m3∶实验组小鼠骨髓细胞中早期凋亡细胞（AnnexinⅤ+PI-）比例（13.10%±5.39%）是对照组（7.16%±2.18%）的1.83倍（P<0.05）；实验组晚期凋亡骨髓细胞（AnnexinⅤ+PI+）比例（7.11%±3.54%）是对照组（4.54%±0.84%）的1.57倍（P<0.05）。（2）900ml/m3苯浓度吸入小鼠组骨髓单个核细胞去乙酰化酶活性[（7.89±2.58）×10-3A值/微克]是正常对照组[（5.00±1.52）×10-3A值/微克）]的1.58倍（P<0.05）。结论 苯吸入可致小鼠骨髓细胞产生凋亡坏死。苯吸入致小鼠骨髓细胞组蛋白去乙酰化酶活性升高。 |
| 中文关键词:CD1小鼠 细胞凋亡 去乙酰化酶 苯 |
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| Influence of Bone Marrow Apoptosis and the Activity of Histone Deacetylase in Benzene Inhalation Muouse |
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| Abstract:Objective To observe the apoptosis and the activity of histone deacetylase(HDAC) in benzene inhalation mouse bone marrow mononuclear cells. Methods Acclimated CD 1 male mice were put in to a self-made benzene inhalation chamber for 6 hours/day, 5 days/week with 300ml/m3 and 900ml/m3 concentrations of benzene and normal air for 12 weeks. The apoptosis of the bone marrow mononuclear cells was detected by flow cytometry.The nuclear proteins were extracted and the activity of histone deacetylase was tested with the colorimetric HDAC assay kit. Results 1.300ml/m3:the (AnnexinⅤ+PI-)bone marrow cells in model group (13.80%±5.31%) was 1.65 times higher than the control group(8.33%±0.61%)(P<0.05), and there was no difference between two groups of (AnnexinⅤ+PI+)cells(P>0.05). 900ml/m3:the (AnnexinⅤ+PI-)bone marrow cells in model group(13.10%±5.39%) was 1.83 times higher than the control group(7.16%±2.18%)(P<0.05). The(AnnexinⅤ+PI+)bone marrow cells in model group(7.11%±3.54%) was 1.57 times higher than the control group(4.54%±0.84%)(P<0.05).2.The HDAC activity of bone marrow mononuclear cells in 900ml/m3 concentrations of model group[(7.89±2.58)×10-3 (A value/μg)] was 1.58 times higher than the control group[(5.00±1.52)×10-3 (A value/μg)] (P<0.05). Conclusion Benzene inhalation in mouse can cause significantly increased apoptosis of bone marrow cells in CD1 mice.The activity of HDAC is increased in bone marrow cells with chronic benzene exposure. |
| keywords:CD1 mice Apoptosis Acetylation Benzene |
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