| E2F2在类风湿关节炎滑膜成纤维样细胞中的表达研究 |
| 投稿时间：2016-09-08 修订日期：2016-09-27 点此下载全文 |
| 引用本文：张蕊,王林,潘继红.E2F2在类风湿关节炎滑膜成纤维样细胞中的表达研究[J].医学研究杂志,2017,46(3):73-77,156 |
| DOI：
10.11969/j.issn.1673-548X.2017.03.019 |
| 摘要点击次数: 1235 |
| 全文下载次数: 767 |
|
| 基金项目:山东省自然科学基金资助项目（ZR2015YL029） |
|
| 中文摘要:目的 研究E2F2在类风湿关节炎滑膜成纤维样细胞（RA FLs）中的表达，分析其表达与类风湿关节炎病理特征的关系，以及调控E2F2表达的机制。方法 采用瞬时转染的方法将E2F2 siRNA转染入RA FLs后，应用RT-PCR 法筛选干扰效率最高的siRNA，并应用MTS法检测干扰E2F2表达后对RA FLs增殖的影响，采用ELISA法检测E2F2下游基因表达的变化。采用RT-PCR法检测分别使用TNF-α和PDTC刺激RA FLs以及共同使用TNF-α和PDTC刺激RA FLs后E2F2的表达。通过染色质免疫沉淀法（ChIP）检测调控E2F2表达的方式。结果 在siE2F2后，与对照组相比E2F2在mRNA水平表达显著降低（P<0.01），细胞增殖能力减弱（P<0.05）。在RA FLs中TNF-α通过NF-κB信号通路调控E2F2表达。结论 在RA FLs中E2F2存在高表达现象，并且E2F2的高表达参与RA的病变过程，TNF-α通过NF-κB信号通路调控E2F2表达，可做为治疗类风湿关节炎的新靶点。 |
| 中文关键词:类风湿关节炎 滑膜成纤维样细胞 E2F2 |
| |
| Review of E2F Transcription Factor 2 Expression in Rheumatoid Arthritis Fibroblast-like Synovial Cells (RA FLs) |
|
|
| Abstract:Objective To study the relationship between E2F2 and the pathological features of RA and the mechanism of E2F2 expression. Methods The interference efficiency was detected by RT-PCR after RA FLs transfected with E2F2 siRNA. The cell proliferation was analyzed by MTS and the expression of numerous genes was tested by ELISA. To analyze the influence of E2F2 in the immune response by RT-PCR, RA FLs were separately treated with TNF-α and PDTC. The way of regulating E2F2 experssion was tested by Chip. Results Compared with negative control groups, the level of E2F2 mRNA were decreased in the cells transfected with E2F2 siRNA(P<0.01). Silencing E2F2 suppresses the proliferation of RA FLs (P<0.05). TNF-α regulates the expression of E2F2 in RA FLs via the NF-κB pathway. Conclusion Up-regulation of E2F2 is associated with the process of RA and TNF-α regulates the expression of E2F2 in RA FLs via the NF-κB pathway. Thus E2F2 might be a potential target for use in the development of new therapeutic approches of RA. |
| keywords:Rheumatoid arthritis Fibroblast-like synovial cells E2F2 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
