| ESM-1改善小鼠MSCs生物学特性的机制研究 |
| 投稿时间：2016-07-03 修订日期：2016-08-25 点此下载全文 |
| 引用本文：朱宗成,盛晓东,周建龙,范韬,金骁琦.ESM-1改善小鼠MSCs生物学特性的机制研究[J].医学研究杂志,2017,46(3):95-98 |
| DOI：
10.11969/j.issn.1673-548X.2017.03.024 |
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| 基金项目:常熟市科技局和卫生局基金资助项目（CS201423） |
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| 中文摘要:目的 探讨内皮细胞特异性分子-1（endothelial cell-specific molecule 1，ESM-1）预处理小鼠骨髓间充质干细胞（bone marrow-derived mesenchymal stem cells，MSCs）后改善其生物学特性的机制。方法 通过不同浓度的ESM-1，对MSCs进行预处理，分析其分泌的细胞凋亡相关因子包括肿瘤坏死因子-α（TNF-α）、肿瘤坏死因子-β（TNF-β）和干细胞生长相关因子∶缺氧诱导因子（HIF）、转化生长因子（TGF-β1）等细胞因子含量的改变情况，以明确ESM-1预处理MSCs改善其生物学特性的机制。结果 通过不同浓度的ESM-1干预MSCs后，各浓度组TNF-α、TNF-β、HIF、TGF-β1含量随着ESM-1干预浓度的升高而升高，各组之间差异有统计学意义（P=0.000）。结论 ESM-1预处理MSCs可提高与炎性反应、细胞凋亡和细胞生长分化相关的多种细胞因子的含量，可能通过多种信号转导通路改善MSCs的生物学特性。 |
| 中文关键词:内皮细胞特异性分子-1 骨髓间充质干细胞 小鼠 |
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| Study on the Mechanism of Improving the Biological Characteristics of Mice MSCs by ESM-1 |
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| Abstract:Objective To explore the possible mechanism about improving the biological characteristics of endothelial cell-specific molecule 1(ESM-1) pretreated with mice MSCs. Methods MSCs was pretreated by different concentration of ESM-1.The possible mechanism was analyzed about improving the biological characteristics of mice MSCs pretreated with ESM-1 through analysis of its secreted cytokines content. The cytokines include apoptosis related factors:TNF-α and TNF-β;stem cell growth factor related:HIF and TGF-β1. Results After the intervention of MSCs with different concentration of ESM-1,the content of cytokines levels including TNF-α,TNF-β,HIF and TGF-β1 increased with the increase of the intervention concentration of ESM-1. The difference between the groups was statistically significant(P=0.000). Conclusion ESM-1 pretreatment with MSCs can improve the content of many cytokines related to inflammatory response, cell apoptosis, cell growth and differentiation, which may improve the biological characteristics of MSCs through a variety of signal transduction pathways. |
| keywords:Endothelial cell-specific molecule 1 Bone marrow-derived mesenchymal stem cells Mice |
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