ESM-1改善小鼠MSCs生物学特性的机制研究
投稿时间:2016-07-03  修订日期:2016-08-25  点此下载全文
引用本文:朱宗成,盛晓东,周建龙,范韬,金骁琦.ESM-1改善小鼠MSCs生物学特性的机制研究[J].医学研究杂志,2017,46(3):95-98
DOI: 10.11969/j.issn.1673-548X.2017.03.024
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作者单位E-mail
朱宗成 215500 常熟, 常熟市第二人民医院心内科 zongchengzhu@163.com 
盛晓东 215500 常熟, 常熟市第二人民医院心内科  
周建龙 215500 常熟, 常熟市第二人民医院心内科  
范韬 215500 常熟, 常熟市第二人民医院心内科  
金骁琦 215500 常熟, 常熟市第二人民医院心内科  
基金项目:常熟市科技局和卫生局基金资助项目(CS201423)
中文摘要:目的 探讨内皮细胞特异性分子-1(endothelial cell-specific molecule 1,ESM-1)预处理小鼠骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,MSCs)后改善其生物学特性的机制。方法 通过不同浓度的ESM-1,对MSCs进行预处理,分析其分泌的细胞凋亡相关因子包括肿瘤坏死因子-α(TNF-α)、肿瘤坏死因子-β(TNF-β)和干细胞生长相关因子∶缺氧诱导因子(HIF)、转化生长因子(TGF-β1)等细胞因子含量的改变情况,以明确ESM-1预处理MSCs改善其生物学特性的机制。结果 通过不同浓度的ESM-1干预MSCs后,各浓度组TNF-α、TNF-β、HIF、TGF-β1含量随着ESM-1干预浓度的升高而升高,各组之间差异有统计学意义(P=0.000)。结论 ESM-1预处理MSCs可提高与炎性反应、细胞凋亡和细胞生长分化相关的多种细胞因子的含量,可能通过多种信号转导通路改善MSCs的生物学特性。
中文关键词:内皮细胞特异性分子-1  骨髓间充质干细胞  小鼠
 
Study on the Mechanism of Improving the Biological Characteristics of Mice MSCs by ESM-1
Abstract:Objective To explore the possible mechanism about improving the biological characteristics of endothelial cell-specific molecule 1(ESM-1) pretreated with mice MSCs. Methods MSCs was pretreated by different concentration of ESM-1.The possible mechanism was analyzed about improving the biological characteristics of mice MSCs pretreated with ESM-1 through analysis of its secreted cytokines content. The cytokines include apoptosis related factors:TNF-α and TNF-β;stem cell growth factor related:HIF and TGF-β1. Results After the intervention of MSCs with different concentration of ESM-1,the content of cytokines levels including TNF-α,TNF-β,HIF and TGF-β1 increased with the increase of the intervention concentration of ESM-1. The difference between the groups was statistically significant(P=0.000). Conclusion ESM-1 pretreatment with MSCs can improve the content of many cytokines related to inflammatory response, cell apoptosis, cell growth and differentiation, which may improve the biological characteristics of MSCs through a variety of signal transduction pathways.
keywords:Endothelial cell-specific molecule 1  Bone marrow-derived mesenchymal stem cells  Mice
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