| CRF及其受体在DSS诱导的实验性结肠炎中的表达 |
| 投稿时间：2016-07-11 修订日期：2016-07-18 点此下载全文 |
| 引用本文：杨丽,郑鹏远,于咏.CRF及其受体在DSS诱导的实验性结肠炎中的表达[J].医学研究杂志,2017,46(3):153-156 |
| DOI：
10.11969/j.issn.1673-548X.2017.03.038 |
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| 中文摘要:目的 研究CRF及其受体在实验性DSS结肠炎中的表达，探讨其在肠道炎症形成中的可能作用。方法 将6~8周的雌性BALB/c小鼠分为正常对照组和实验组，建立DSS诱导的实验性结肠炎小鼠模型，小鼠进行DAI和组织病理学评分。免疫荧光检测小鼠肠黏膜组织中CRF1受体和CRF2受体的表达。Western blot法检测小鼠肠黏膜组织中CRF、CRF1和CRF2受体的表达。结果 根据肠黏膜DAI评分和组织学评分提示DSS组肠黏膜组织炎症明显，造模成功。免疫荧光检测显示CRF1受体在DSS组和正常肠黏膜组织中表达差异无统计学意义（P>0.05），而DSS组小鼠肠黏膜组织中CRF2受体表达明显高于对照组（P<0.05）且主要分布在肠上皮细胞和固有层单个核细胞中。Western blot法检测显示CRF1受体在DSS组和正常肠黏膜组织中表达差异无统计学意义（P>0.05），而DSS组小鼠肠黏膜组织中CRF和CRF2受体表达明显高于对照组（P<0.05）。结论 DSS结肠炎模型周围肠黏膜炎症组织中存在着CRF和CRF2受体的表达增高，而这种增高可能与肠道炎症发生有关。 |
| 中文关键词:促肾上腺皮质激素释放因子 结肠炎 CRF受体 |
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| CRF and CRF Receptors Expression in DSS Colitis Model in Mice |
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| Abstract:Objective To detect the expression of CRF and CRF receptors in colonic mucosa of DSS induced colitis in mice model and to study the effect of CRF and CRF receptors on the development. Methods Six to eight weeks healthy female BALB/c mice were divided into control group and DSS group. Setting up DSS colitis model and colitis was evaluated by the disease activity index(DAI) and histological score. The immunofluorescence technique was used to assay the CRF1 and CRF2 receptors expression in colonic mucosa. The expression of CRF and CRF receptors protein were analyzed by western blotting. Results DSS colitis was set up successfully with significant inflammation in colonic mucosa by the disease activity index(DAI) and histological score. Immunofluorescenee staining evidenced that expression of CRF1 receptor in DSS colitis group has no significant deviation compared to control group(P>0.05), while the expression of CRF2 receptor was elevated in DSS colitis group compared to control group (P<0.05). CRF2 receptor was localized in epithelial cells and mononuclear cells in the lamina propria.The levels of CRF and CRF2 receptor protein by western blotting were higher in in DSS colitis group compared to control group (P<0.05).The level of CRF1 receptor protein in DSS colitis group had no significant deviation compared to control group(P>0.05). Conclusion The higher expression of CRF and CRF2 in colonic mucosa of DSS colitis may participate in the development of colitis. |
| keywords:Corticotropin-releasing factor Colitis CRF receptor |
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