| α-半水硫酸钙骨修复材料修复犬股骨髁缺损的实验研究 |
| 投稿时间：2016-08-22 修订日期：2016-09-07 点此下载全文 |
| 引用本文：陈克伟,周英勇,顾运涛,程少文,孟珠龙,刘建莉,彭磊.α-半水硫酸钙骨修复材料修复犬股骨髁缺损的实验研究[J].医学研究杂志,2017,46(4):52-56 |
| DOI：
10.11969/j.issn.1673-548X.2017.04.014 |
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| 基金项目:浙江省科技计划项目（2010C34006）；海南省自然科学基金资助项目（514216）；海南省科技合作专项基金资助项目（KJHZ2015-05） |
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| 中文摘要:目的 研究α-半水硫酸钙人工骨对犬股骨缺损的修复作用。方法 18只健康成年雄性家犬，建立双侧股骨下端骨缺损模型，随机分成3组，每组6只。A 组：右侧植入自制α-半水硫酸钙，左侧植入Osteoset®片；B 组：右侧植入自制α-半水硫酸钙，左侧骨缺损处不植入任何材料作为空白对照；C组：右侧骨缺损处不植入任何材料作为对照，左侧植入Osteoset®片，分笼饲养，分别于术后4、8、12周处死动物，行大体观察、X 线观察、HE染色组织学观察、骨密度检测、骨组织计量分析。结果 X线观察显示，骨缺损处植入的α-半水硫酸钙和Osteoset®人工骨逐渐降解吸收，被新生的骨组织取代，同期空白对照侧仅在骨缺损边缘处仅见少量新生骨组织。组织学观察显示，各期α-半水硫酸钙和Osteoset®人工骨植入区边缘可见分布密集的骨小梁和较多核深染的骨细胞，而同期空白对照侧缺损处骨小梁较稀疏，骨细胞数量较少。骨密度检测显示，各期两种人工骨植入部位骨密度无明显差别，均明显高于空白对照组。骨组织计量分析显示，各期两种人工骨材料植入区新生骨量无明显差别，但均显著多于同期空白对照组新生骨量。结论 α-半水硫酸钙人工骨成骨能力与Osteoset®人工骨一样，能够修复包容性骨缺损，是一种理想的人工骨植入材料。 |
| 中文关键词:α-半水硫酸钙 人工骨 骨缺损 骨修复 |
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| Experimental Study on Repair of Femoral Defects in Dogs with α-Calcium Sulphate Hemihydrate Artificial Bone |
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| Abstract:Objective To study the effect of α-calcium sulphate hemihydrate artificial bone on the repair of canine femoral defect. Methods The model of double lower limb femoral condyle defect,was established with 18 healthy adult male dogs then they were randomly divided into three groups, each with 6.Group A: the self-made α-calcium sulphate hemihydrate was implanted in the right femur, Osteoset® was implanted in the left femur;Group B: the self-made α-calcium sulphate hemihydrate was implanted in the right femur, and left femur implanted into nothing, as blank control group.Group C: the right side of the femur was not implanted in any material as a blank control group,Osteoset® was implanted in the left side of the femur.Animals were assigned to the cage breeding alone. After 4 weeks, 8 weeks, 12 weeks, the animals were killed, the materials were taken out for general observation, X-ray observation, HE staining histological observation, bone density measurement. Results X-ray observation results showed that in the bone defect, the α-calcium sulphate hemihydrate and Osteoset® artificial bone were gradually degraded and absorbed, which was replaced by the new bone tissue. At the same time, only a small amount of newly formed bone tissue was seen at the edge of the blank control group. Histological observation showed that in the edge of the α-calcium sulphate hemihydrate and Osteoset® artificial bone implant area,there were dense distribution of trabecular bone and many nuclear hyperchromatic bone cells were found.While in the same period, the trabecular bone was more sparse and the number of bone cells was less in the blank control side.Bone mineral density detection resutls showed that the bone mineral density of the two kinds of artificial bone implant area were significantly higher than the blank control group. Bone tissue measurement analysis showed that the number of newly formed bone had no significant difference in the region of artificial bone implantation, but it was significantly more than that in the control group. Conclusion The treatment of delayed union and nonunion of tibial fracture by percutaneous autologous bone marrow transplantation is satisfactory, and it is worthy of clinical application. |
| keywords:α-Calcium sulphate hemihydrate Artificial bone Bone defect Bone repair |
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