| 羊栖菜多糖对TGF-β1诱导的大鼠肺成纤维细胞的影响 |
| 投稿时间：2016-07-07 修订日期：2016-07-29 点此下载全文 |
| 引用本文：吴华勇,方红龙,罗建,王丹琼,张伟文.羊栖菜多糖对TGF-β1诱导的大鼠肺成纤维细胞的影响[J].医学研究杂志,2017,46(4):74-77 |
| DOI：
10.11969/j.issn.1673-548X.2017.04.019 |
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| 基金项目:浙江省卫生厅基金资助项目（2014KYB302） |
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| 中文摘要:目的 研究羊栖菜多糖对TGF-β1诱导的大鼠肺成纤维细胞的影响，探讨其抗肺纤维化作用及机制。方法 将实验分成对照组、TGF-β1组、TGF-β1+不同剂量羊栖菜多糖组（25、50、100μg/ml），采用MTT法检测细胞增殖，RT-PCR法检测Smad 3、Smad 7、α-SMA、Ⅰ型胶原mRNA的表达，ELISA法检测Ⅰ型胶原蛋白的表达， Western blot法检测α-SMA的表达。结果 TGF-β1能诱导肺成纤维细胞增殖及表达Smad3 mRNA、Smad7 mRNA、Ⅰ型胶原、α-SMA（P < 0.05）。羊栖菜多糖可以不同程度抑制TGF-β1诱导的肺成纤维细胞的增殖以及Smad3mRNA、Ⅰ型胶原和α-SMA的表达，且呈剂量依赖性（P < 0.05），能促进TGF-β1诱导的肺成纤维细胞表达Smad7 mRNA， 呈剂量依赖性（P < 0.05）。结论 在离体细胞，羊栖菜多糖能抑制TGF-β1诱导的肺成纤维细胞增殖、分化与表达，其机制可能与其下调Smad3，促进Smad 7表达从而抑制TGF-β/Smads信号通路有关。 |
| 中文关键词:羊栖菜多糖 肺成纤维细胞 TGF-β/Smads信号通路 |
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| Effects of Sargassum Fusiforme Polysaccharide on Transforming Growth Factor β1-induced Rat Lung Fibroblasts |
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| Abstract:Objective To investigate the effects of Sargassum fusiforme polysaccharide to the transforming growth factor beta1(TGF-β1)-induced rat lung fibroblast cells in vitro,and to evaluate the anti-fibrotic mechanism of SFPS. Methods Cultured rat lung fibroblast cells were divided into a control group,a TGF-β1-treated group and TGFβ1(5ng/ml) plus different dosage SFPS-treated groups(TGF-β1 5ng/ml+SFPS 25μg/ml group; TGF-β1 5ng/ml+SFPS 50μg/ml group; TGF-β1 5ng/ml+SFPS 100μg/ml group). Cell proliferation was assessed by MTT colorimetric assay. The levels of collagen type Ⅰ mRNA and protein was measured by RT-PCR and ELISA. RT-PCR and Western blot were used to detect the expression of α-smooth muscle actin mRNA and protein. RT-PCR were used to detect the expression of Smad3,Smad7mRNA. Results TGF-β1 induced proliferation and the expressions of Smad3,Smad7mRNA, collagen type Ⅰ, α-smooth muscle actin of lung fibroblasts (P < 0.05). SFPS prevented TGF-β1-induced proliferation and the expressions of Smad3mRNA, collagen type Ⅰ, α-smooth muscle actin of lung fibroblasts, this prevention effect was dose-dependent (P < 0.05). SFPS increased TGF-β1-induced expressions of Smad7 mRNA this effect was also dose-dependent (P < 0.05). Conclusion In vitro cell, SFPS can inhibit TGF-β1-induced lung fibroblast proliferation, differentiation and expression.The mechanism may be related to reduced Smad3, promoted Smad7 thereby inhibiting TGF-β/Smads signaling pathway. |
| keywords:Sargassum fusiforme polysaccharide Lung fibroblasts TGF-β/Smads signaling |
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