| HBV相关肝病患者外周血NK细胞PD-1、Tim-3表达及意义 |
| 投稿时间：2016-09-01 修订日期：2016-09-26 点此下载全文 |
| 引用本文：赵小瑜,周妮,汤永志,林希,陈华忠,朱坚胜.HBV相关肝病患者外周血NK细胞PD-1、Tim-3表达及意义[J].医学研究杂志,2017,46(5):51-54,59 |
| DOI：
10.11969/j.issn.1673-548X.2017.05.014 |
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| 基金项目:浙江省医药卫生科技计划项目（2012KYB237） |
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| 中文摘要:目的 了解HBV相关肝病患者外周血NK细胞表面PD-1、Tim-3的表达情况，探讨PD-1和Tim-3的表达与血清HBV-DNA载量的相关情况。方法 选择HBV感染相关肝病患者74例，其中CHB患者30例，LC患者24例，HCC患者20例，健康对照18例。应用流式细胞术检测NK细胞表面PD-1、Tim-3表达，荧光定量PCR检测HBV-DNA量，ELISA法检测细胞因子分泌水平。统计各组患者PD-1和Tim-3的表达率，比较各组差异，分析其与病情的相关性。结果 CHB、LC、HCC组外周血NK细胞表面PD-1和Tim-3的表达率分别为34.46%±7.31%、59.09%±12.35%、61.49%±15.26%和19.18%±6.05%、28.64%±11.20%、31.24%±11.85%，均高于对照组（P<0.01），LC组和HCC组明显高于CHB组（P<0.01），LC组和HCC组比较无统计学差异。在CHB组，PD-1的表达率与HBV-DNA呈负相关关系（R=-0.437，P=0.033）。CHB、LC、HCC各组血清中细胞因子TNF-α和IFN-γ水平分别为0.66±0.63、0.75±0.66、0.85±0.71和1.76±0.88、1.83±1.23、1.60±0.53，均低于健康对照组（P<0.01）。结论 HBV相关肝病患者NK细胞表面PD-1、Tim-3表达均增加，且与病情严重程度有关，增加表达的PD-1与血清HBV-DNA载量存在负相关性，HBV肝病各组血清TNF-α、IFN-γ分泌减少。 |
| 中文关键词:乙型肝炎病毒 自然杀伤细胞 程序性死亡受体-1 T细胞免疫球蛋白黏蛋白分子-3 |
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| Expression and Meaning of PD-1,Tim-3 on NK in HBV Related Liver Disease |
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| Abstract:Objective To quantitative analysis the expression variation of PD-1, Tim-3 on HBV-related liver disease patients peripheral blood NK cell. To investigate the correlation of serum HBV-DNA loads and the expression of PD-1, Tim-3. Methods All of 92 subjects including 30 CHB, 24 LC, 20 HCC and 18 healthy adults were enrolled in present study. The expression of PD-1 and Tim-3 were detected by flow cytometry. The quantity of HBV-DNA were obtained by fluorescence quantitative PCR, and the concentrations of cytokines were assayed by enzyme linked immunosorbent assay(ELISA).The expression of PD-1, Tim-3 in each group, was Calculated and compared the differences, analyzed its correlation with disease progression. Results In group of CHB, LC, HCC, expression of PD-1 and Tim-3 on peripheral blood NK cell were 34.46%±7.31%, 59.09%±12.35%, 61.49%±15.26% and 19.18%±6.05%,28.64%±11.20%,31.24%±11.85% respectively, which were higher than control group (P<0.01). Group of LC and HCC were higher than CHB group (P<0.01), but no significant difference were found between LC and HCC group. In the group of CHB, the expression of PD-1 and HBV-DNA loads was negatively correlated(R=-0.437, P=0.033). Compared with the group of control, the concentration of TNF-α and IFN-γ decreased in the group of CHB, LC, HCC 0.66±0.63, 0.75±0.66, 0.85±0.71 and 1.76±0.88, 1.83±1.23, 1.60±0.53, (P<0.01). Conclusion The expression of PD-1, Tim-3 on NK cell were increased in HBV-related liver disease patients, and the increased expression of PD-1 was negatively correlated with serum HBV-DNA loads. Concentration of TNF-α,IFN-γ were decreased in HBV related liver disease patients. |
| keywords:Hepatitis B virus Natural killer cell Programmed death-1 T cell immunoglobulin-and mucin-domain-containing molecule-3 |
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