| 高脂饮食大鼠肾脏HMGCS2表达及调控研究 |
| 投稿时间：2016-10-23 修订日期：2016-11-01 点此下载全文 |
| 引用本文：廖丹,章小艳,童晓清,斯彩娟,余小龙,束龙,张伦,王卫光,高蔚,郑培奋.高脂饮食大鼠肾脏HMGCS2表达及调控研究[J].医学研究杂志,2017,46(5):66-69 |
| DOI：
10.11969/j.issn.1673-548X.2017.05.018 |
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| 作者 | 单位 | E-mail | | 廖丹 | 310013 杭州, 浙江医院 | 376599136@qq.com | | 章小艳 | 310013 杭州, 浙江医院 | | | 童晓清 | 310013 杭州, 浙江医院 | | | 斯彩娟 | 310013 杭州, 浙江医院 | | | 余小龙 | 310013 杭州, 浙江医院 | | | 束龙 | 310013 杭州, 浙江医院 | | | 张伦 | 310013 杭州, 浙江医院 | | | 王卫光 | 310013 杭州, 浙江医院 | | | 高蔚 | 310013 杭州, 浙江医院 | | | 郑培奋 | 310013 杭州, 浙江医院 | kuaidou09@163.com |
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| 基金项目:浙江省卫生厅厅级培育项目（2014PYA002） |
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| 中文摘要:目的 探讨高脂饮食大鼠肾脏3-羟基-3甲基戊二酰辅酶A合成酶2（HMGCS2）表达及可能调控机制。方法 断乳Wistar 雄性大鼠27只分为3组：正常对照组（CON）、高脂组（HFG）、绿茶多酚（green tea polyphenols，GTPs）组（G）。CON组大鼠喂养普通饲料，HFG组及GTPs组给予高脂饲料，GTPs组同时自由摄食绿茶多酚浓度为1.6g/L水溶液。26周后测定空腹血糖（FPG）、血清胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）。免疫印迹法测定肾脏HMGCS2和Sirt3蛋白表达水平。结果 与CON组比较，HFG组大鼠体重和脂肪系数升高，血清FPG、TC、TG、LDL-C/HDLC均升高，差异有统计学意义（P<0.05）；与HFG组比较，GTPs降低大鼠体重和脂肪系数，差异有统计学意义（P<0.05），GTPs组血清FPG、TC、TG、LDL-C/HDLC均不同程度降低，差异有统计学意义（P<0.05）。与CON组比较，肾脏组织HMGCS2的表达降低，差异有统计学意义（P<0.05）；与HFG组比较，GTPs组增加肾脏HMGCS2的表达，差异有统计学意义（P<0.05）。高脂饮食、正常饮食和GTPs干预后，大鼠肾脏组织Sirt3蛋白表达差异无统计学意义。结论 高脂饮食可降低肾脏组织HMGCS2表达，绿茶多酚可逆转此效应；高脂饮食及绿茶多酚对肾脏组织HMGCS2表达的调控不经Sirt3途径。 |
| 中文关键词:高脂饮食 3-羟基-3-甲基戊二酰辅酶A合成酶2（HMGCS2） 绿茶多酚（GTPs） |
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| Expression of Renal HMGCS2 in Rats Fed With High Fat Diet |
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| Abstract:Objective To study the expression of renal HMGCS2 in rats fed with high fat diet and its mechanism. Methods Male Wistar rats were divided into three groups: normal control group (CON), high fat group (HFG), GTPs group (G),with 9 in each group. The CON group rats were fed a normal diet, the HFG group fed a high fat diet while the GTPs group fed the same high fat diet with drinking water containing GTPs. After 26 weeks, serum concentrations of fasted glucose, total cholesterol, triglyceride, LDL and HDL were measured by ELX 800 using their corresponding commercial kits. The expressions of HMGCS2 and Sirt3 were determined by western blot. Results Serum levels of TC, TG, LDL-C, FPG content in HFG group were higher than those of CON group (P<0.05). Compared with HFG group, serum TC, TG, LDL-C levels and FPG in GTPs group were decreased. The expressions of renal HMGCS2 protein were decreased in HFG group. In GTPs treated group, the expressions of HMGCS2 were increased in contrast to HFG group. Meanwhile, the expressions of Sirt3 had no significance among the three groups. Conclusion The expression of renal HMGCS2 in rats fed with high fat diet was decreased. Green tea polyphenols may protect the kidneys through regulating blood glucose and lipids, and up-regulation of HMGCS2 protein expression. |
| keywords:High fat diet HMGCS2 Green tea polyphenols |
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