BrdU标记法探讨糖尿病大鼠H2S与细胞外基质积聚的关系
投稿时间:2016-09-29  修订日期:2016-10-17  点此下载全文
引用本文:陈燊,黄雯,张梅,张国娟.BrdU标记法探讨糖尿病大鼠H2S与细胞外基质积聚的关系[J].医学研究杂志,2017,46(5):112-115
DOI: 10.11969/j.issn.1673-548X.2017.05.028
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作者单位E-mail
陈燊 100060 首都医科大学附属北京同仁医院肾内科  
黄雯 100060 首都医科大学附属北京同仁医院肾内科 huangw@trhos.com 
张梅 100060 首都医科大学附属北京同仁医院肾内科  
张国娟 100060 首都医科大学附属北京同仁医院肾内科  
基金项目:首都医科大学附属北京同仁医院基金资助项目(2014-FDZ-026)
中文摘要:目的 探讨硫化氢(hydrogen sulfide,H2S)与糖尿病肾病(diabetic nephropathy,DN)大鼠肾细胞外基质过度积聚的关系。方法 21只SD大鼠随机分成正常对照组(对照组)、糖尿病肾病组(DN组)、硫化氢干预组(DN+H2S组)。链脲佐菌素(STZ)法建立糖尿病大鼠模型,DN+H2S组给予NaHS治疗8周,其他组给予同等剂量的0.9%生理盐水治疗。观察3组大鼠肾脏病理改变、BrdU阳性细胞和α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)分布差异。结果 与对照组比较,DN组大鼠尿蛋白量、相对肾重均明显增多,病理呈典型的DN改变,BrdU阳性细胞比值、肾小球α-SMA表达和分布显著增多(P<0.01);应用NaHS治疗后,大鼠肾脏DN改变明显减轻(P<0.01),BrdU阳性细胞比值、肾小球α-SMA表达和分布与正常对照组比较,差异无统计学意义(P>0.05)。结论 H2S可以抑制DN大鼠肌性成纤维细胞增殖,减少α-SMA表达和分布,减缓DN大鼠的肾小球细胞外基质积聚。
中文关键词:硫化氢  糖尿病肾病  α平滑肌肌动蛋白
 
Investigation on the Relationship of H2S and the Accumulation of Extracellular Matrix of the Diabetic Nephropathic Rats By BrdU labeling method
Abstract:Objective To explore the relationship of hydrogen sulfide (H2S) and the extracellular matrix of the diabetic nephropathic rats' glomerulars. Methods Twenty-one male rats(Sprague-Dawley) were randomly divided into Control group(n=7), DN group(n=7) and DN+H2S group (n=7). Diabetes was induced in the rats except Control group rats by STZ(60mg/kg). The rats of the DN+H2S group were injected with NaHS every day,and rats of other groups were injected with NS. Eight weeks later, three groups were compared in proteinuria of 24 hours, KW/BW, the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars. Results Compared with Control group, DN group rats were significantly increased in proteinuria of 24 hours and KW/BW(P<0.01). Kidney biopsy in DN groups rats were typical DN pathological manifestation. In treatment with NaHS, the DN rats in the DN+H2S group were decreased in proteinuria of 24 hours, KW/BW, the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars(P<0.01),and was similar with the control group in the pathology of renal. Conclusion The H2S control the multiplication of myofibroblast, and were decreased in the distribution of α-SMA in glomerulars. The H2S treatment may put off the progress of the extracellular matrix in the DN rats' glomerulars.
keywords:Hydrogen sulfide  Diabetic nephropathy  α-Smooth muscle actin(α-SMA)
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