| BrdU标记法探讨糖尿病大鼠H2S与细胞外基质积聚的关系 |
| 投稿时间：2016-09-29 修订日期：2016-10-17 点此下载全文 |
| 引用本文：陈燊,黄雯,张梅,张国娟.BrdU标记法探讨糖尿病大鼠H2S与细胞外基质积聚的关系[J].医学研究杂志,2017,46(5):112-115 |
| DOI：
10.11969/j.issn.1673-548X.2017.05.028 |
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| 基金项目:首都医科大学附属北京同仁医院基金资助项目（2014-FDZ-026） |
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| 中文摘要:目的 探讨硫化氢（hydrogen sulfide，H2S）与糖尿病肾病（diabetic nephropathy，DN）大鼠肾细胞外基质过度积聚的关系。方法 21只SD大鼠随机分成正常对照组（对照组）、糖尿病肾病组（DN组）、硫化氢干预组（DN+H2S组）。链脲佐菌素（STZ）法建立糖尿病大鼠模型，DN+H2S组给予NaHS治疗8周，其他组给予同等剂量的0.9%生理盐水治疗。观察3组大鼠肾脏病理改变、BrdU阳性细胞和α平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）分布差异。结果 与对照组比较，DN组大鼠尿蛋白量、相对肾重均明显增多，病理呈典型的DN改变，BrdU阳性细胞比值、肾小球α-SMA表达和分布显著增多（P<0.01）；应用NaHS治疗后，大鼠肾脏DN改变明显减轻（P<0.01），BrdU阳性细胞比值、肾小球α-SMA表达和分布与正常对照组比较，差异无统计学意义（P>0.05）。结论 H2S可以抑制DN大鼠肌性成纤维细胞增殖，减少α-SMA表达和分布，减缓DN大鼠的肾小球细胞外基质积聚。 |
| 中文关键词:硫化氢 糖尿病肾病 α平滑肌肌动蛋白 |
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| Investigation on the Relationship of H2S and the Accumulation of Extracellular Matrix of the Diabetic Nephropathic Rats By BrdU labeling method |
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| Abstract:Objective To explore the relationship of hydrogen sulfide (H2S) and the extracellular matrix of the diabetic nephropathic rats' glomerulars. Methods Twenty-one male rats(Sprague-Dawley) were randomly divided into Control group(n=7), DN group(n=7) and DN+H2S group (n=7). Diabetes was induced in the rats except Control group rats by STZ(60mg/kg). The rats of the DN+H2S group were injected with NaHS every day,and rats of other groups were injected with NS. Eight weeks later, three groups were compared in proteinuria of 24 hours, KW/BW, the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars. Results Compared with Control group, DN group rats were significantly increased in proteinuria of 24 hours and KW/BW(P<0.01). Kidney biopsy in DN groups rats were typical DN pathological manifestation. In treatment with NaHS, the DN rats in the DN+H2S group were decreased in proteinuria of 24 hours, KW/BW, the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars(P<0.01),and was similar with the control group in the pathology of renal. Conclusion The H2S control the multiplication of myofibroblast, and were decreased in the distribution of α-SMA in glomerulars. The H2S treatment may put off the progress of the extracellular matrix in the DN rats' glomerulars. |
| keywords:Hydrogen sulfide Diabetic nephropathy α-Smooth muscle actin(α-SMA) |
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