| SSR在心肌重构过程中的表达改变 |
| 投稿时间：2016-09-24 修订日期：2016-10-23 点此下载全文 |
| 引用本文：肖杨,吴青青,唐其柱.SSR在心肌重构过程中的表达改变[J].医学研究杂志,2017,46(5):124-127 |
| DOI：
10.11969/j.issn.1673-548X.2017.05.031 |
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| 中文摘要:目的 探讨SSR在心肌重构过程中的表达改变。方法 采用冠状动脉左前降支结扎术建立小鼠心肌梗死后的心肌重构模型、异丙肾上腺素（ISO）皮下注射2周建立小鼠急性心脏损伤模型；胸主动脉结扎术（aortic banding，AB）建立小鼠心肌肥厚的模型。采用RT-PCR检测各种心肌重构模型中SSR转录水平的改变。结果 SSR亚单位1（SSR1）和3（SSR3）在小鼠心肌梗死（miocardial infarction，MI）术后2周表达明显降低（P<0.05）；在ISO诱导的急性心脏损伤后2周表达降低（P<0.05）；在AB术后1周表达降低（P<0.05）。然而SSR1和SSR3的表达在AB术后2周开始增高（P<0.05），持续到AB术后8周（P<0.05）。结论 SSR1和SSR3的表达在不同模型的心肌重构中均发生明显改变，且呈现动态变化，提示其可能参与心肌重构的发生、发展。 |
| 中文关键词:信号序列受体 心肌梗死 异丙肾上腺素 胸主动脉缩窄术 心肌重构 |
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| Expression Alteration of SSR in the Process of Cardiac Remodeling |
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| Abstract:Objective To investigate the expression changes of SSR in the process of cardiac remodeling. Methods Myocardial infarction (MI) was induced by left anterior descending coronary artery ligation in mice to establish cardiac remodeling model. Mice subjected to isoproterenol (ISO) subcutaneous injection for 2 weeks to establish acute cardiac injury model. Mice subjected to aortic banding (AB) to establish a mouse model of cardiac hypertrophy. RT-PCR was used to detect the expression change of SSR in various cardiac remodeling models. Results The expression levels of SSR subunit 1 (SSR1) and 3 (SSR3) were significantly decreased in mice after 2 weeks of MI (P<0.05), and were also decreased in acute cardiac injury induced by 2 weeks of ISO injection (P<0.05), and reduced after1 week of AB operation (P<0.05). However, the expression of SSR1 and SSR3 increased at 2 weeks after AB (P<0.05), and sustained to 8 weeks after AB (P<0.05). Conclusion The expression of SSR3 and SSR1 in different models of cardiac remodeling were significantly changed, and showed dynamic changes, suggesting that it may participate in the occurrence and development of cardiac remodeling. |
| keywords:Signal sequence receptor Myocardial infarction Isoproterenol Aortic banding Cardiac remodeling |
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