| 苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响 |
| 投稿时间：2016-10-23 修订日期：2016-11-07 点此下载全文 |
| 引用本文：秦聪,张杰,张礼军.苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响[J].医学研究杂志,2017,46(6):44-47 |
| DOI：
10.11969/j.issn.1673-548X.2017.06.012 |
| 摘要点击次数: 1245 |
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| 基金项目:国家自然科学基金资助项目（81470923） |
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| 中文摘要:目的 观察苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响并探讨其作用机制。方法 实验分组如下，A组：膀胱癌EJ细胞不作处理；B组：加入苦豆碱浓度25μmol/L；C组：加入苦豆碱浓度50μmol/L；D组：加入苦豆碱浓度100μmol/L。通过CCK8检测各组细胞的存活率，通过流式细胞术检测各组细胞凋亡率，通过Western blot法检测各组细胞Bcl-2、Bax、p-Erk1/2蛋白的表达水平。结果 CCK8结果显示，B、C、D组较A组细胞存活率降低（P<0.05）；流式细胞术结果显示：B、C、D组较A组细胞凋亡率增加（P<0.05）；Western blot结果显示：B、C、D组较A组Bcl-2、p-Erk1/2表达水平降低（P<0.05），Bax表达水平增高（P<0.05）。结论 苦豆碱可降低膀胱癌EJ的存活率，诱导其凋亡，其作用机制可能是通过抑制Erk1/2的磷酸化而实现的。 |
| 中文关键词:苦豆碱 膀胱癌 增殖 凋亡 |
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| Effects of Aloperine on Proliferation and Apoptosis of Bladder Cancer EJ Cells |
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| Abstract:Objective To investigate the effect of aloperine on the proliferation and apoptosis of bladder cancer EJ cells and to explore its mechanism. Methods The experimental group was as follows:bladder cancer EJ cells were not treated (group A); 25μmol/L aloperine was added (group B); 50μmol/L aloperine was added (group C). 100μmol/L aloperine was added (group D). The survival rate of the cells was detected by CCK8. The apoptosis rate was detected by flow cytometry. The expression levels of Bcl-2, Bax, p-Erk1/2 protein were detected by Western blot. Results CCK8 results showed that:B, C, D group compared with A group, the cell survival rate were decreased (P<0.05). The results of flow cytometry showed that the apoptosis rate of B, C and D group was higher than that of A group (P<0.05). Western blot results showed that:B, C, D group compared with A group the expression of Bcl-2 and p-Erk1/2 levels were reduced (P<0.05), the expression of Bax level was increased (P<0.05). Conclusion The survival rate of EJ in bladder cancer can be reduced by aloperine and induce its apoptosis, and inhibiting the phosphorylation of Erk1/2 may be the mechanism. |
| keywords:Aloperine Bladder cancer Proliferation Apoptosis |
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