| 不同分割照射方式对MDA-MB-231移植瘤增殖活性的影响 |
| 投稿时间：2016-11-05 修订日期：2016-11-09 点此下载全文 |
| 引用本文：辛科道,章青.不同分割照射方式对MDA-MB-231移植瘤增殖活性的影响[J].医学研究杂志,2017,46(7):57-61 |
| DOI：
10.11969/j.issn.1673-548X.2017.07.014 |
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| 基金项目:上海市卫生和计划生育委员会基金资助项目（面上项目）（20134051） |
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| 中文摘要:目的 以相同剂量下，研究单次大剂量照射对MDA-MB-231乳腺癌移植瘤的生物学效应。方法 建立MDA-MB-231乳腺癌移植瘤模型，待移植瘤直径达8~9mm，随机分为0Gy组（空白对照组）、8Gy组（8Gy/1f/1d）、10Gy组（10Gy/1f/1d）、4×2Gy组（8Gy/4f/4d）、2Gy×5（10Gy/5f/5d）组。每3天观察肿瘤体积变化，绘制移植瘤生长曲线。照射实验组（除对照组）裸鼠分别于放疗结束后第7天、14天行18F-FDG PET/CT检查，另取各实验组裸鼠分别于放疗结束后7天、14天处死，收集肿瘤组织行免疫组化测定Ki-67的表达。结果 单次大剂量和常规分割照射均能抑制MDA-MB-231移植瘤生长，以单次大剂量10Gy组最为明显（P<0.05）。且在同等剂量条件下，单次大剂量组在放疗后第7、14天PET/CT检查显示移植瘤SUVmax值均低于常规分割照射组（P<0.05），Ki-67阳性细胞百分比单次大剂量组也低于同剂量常规分割组（P<0.05）。结论 在同等剂量情况下，单次大剂量放疗较常规分割放疗对MDA-MB-231乳腺癌细胞增殖活性的抑制更为显著，同时18F-FDG PET/CT在一定程度上可以反应早期放疗后MDA-MB-231乳腺癌细胞增殖活性。 |
| 中文关键词:乳腺癌 单次大剂量照射 Ki-67 PET/CT |
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| Bio-Effects of Different Irradiation Regimens on Xenograft of MDA-MB-231 Breast Carcinoma |
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| Abstract:Objective To explore the bio-effects of high single-dose irradiation on MDA-MB-231 xenografts under the same radiation dose. Method Female BALB/c-nu mice bearing 8-9 mm MDA-MB-231 xenografts were randomized into 5 groups:0Gy group(blank control group),high single-dose 8Gy group(8Gy/one fraction/day), high single-dose 10Gy group(10Gy/one fraction/day),conventional radiation 2Gy×4 group(8Gy/4 fraction/4d),conventional radiation 2Gy×5 group(10Gy/5 fraction/5d). The volumes of gross tumors on nude mice were observed every three days. The tumor growth curve of transplanted tumor were also drawn.The 18F-FDG PET/CT imaging of mice bearing MDA-MB-231 xenografts from all radiation groups were performed with detecting the Ki-67 expressions of tumor cells by immunohistochemistry at 7days and 14days after final irradiation. Results Compared to control group,the tumor growth of all radiation groups were delayed after radiation,especially single high-dose 10Gy groups (P<0.05). At 7days and 14days after final radiation, the SUVmax (the maximal standardized uptake value) of xenografts in high single-dose groups was lower than that in conventional fractional groups (P<0.05) under the same radiation dose. And the percent of Ki-67 positive cells after high single-dose irradiation were less than fractioned irradiation at 7,14days after final radiation (P<0.05). Conclusion At the same dose, high single-dose irradiation inhibits the growth of MDA-MB-231 xenografts more than conventional fraction irradiation.PET/CT also could evaluated the early changes of proliferation activities of MDA-MB-231 breast cancer cells after irradiation. |
| keywords:Breast cancer High single-dose radiation Ki-67 PET/CT |
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