| 维生素D受体基因多态性与青藏高原藏族儿童结核病的对照研究 |
| 投稿时间：2016-11-21 修订日期：2016-12-22 点此下载全文 |
| 引用本文：张弢,张艳虹,朱国龙,李天如,王淼舟.维生素D受体基因多态性与青藏高原藏族儿童结核病的对照研究[J].医学研究杂志,2017,46(8):79-82,90 |
| DOI：
10.11969/j.issn.1673-548X.2017.08.020 |
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| 基金项目:青海省科技计划项目（2013-Z-740） |
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| 中文摘要:目的 探讨维生素D受体基因两种多态现象与青藏高原藏族儿童结核发病间的关系。方法 聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）分析的方法分别对162例藏族儿童活动性肺结核患者及167例有分枝杆菌接触史的同民族健康者进行维生素D受体基因ApaⅠ、BsmⅠ多态性的基因型，根据不同基因型对样本分组进行统计学分析以研究两种多态性与青藏高原藏族儿童肺结核易感性的关系。结果 病例组中，AA、Aa与aa 3种基因型别为10例（6.2%）、72例（44.5%）、80例（49.4%），健康对照组的相应基因型则为22例（13.2%）、79例（47.3%）、66例（39.5%），病例组aa基因型频率高于对照组（OR=1.493，95% CI：0.964~2.312，P<0.05）。病例组中，BB、Bb、bb 3种基因型分别为2例（1.2%）、24例（14.8%）、136例（83.9%），健康对照组的相应基因型则为1例（0.6%）、20例（11.9%）、146例（87.4%），χ2检验表明两组的基因型频率差异无统计学意义（OR=1.009，95% CI：0.560~1.816，P>0.05）。结论 维生素D受体的ApaⅠ的多态性与高原藏族儿童肺结核易感性相关，其aa基因型可能是发病风险因素，而BsmⅠ的多态性则无关。 |
| 中文关键词:维生素D受体 青藏高原 藏族儿童 肺结核 |
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| A Case-control Study on the Vitamin D Receptor Gene Polymorphisms and Susceptibility to Pulmonary Tuberculosis (PTB) in Tibetan Children at Qinghai-Tibetan Plateau |
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| Abstract:Objective To study the relationship between Apa I and Bsm I polymorphisms of the VDR gene and the susceptibility of PTB in Tibetan Children at Qinghai-Tibetan plateau. Methods Two polymorphisms of the VDR gene Apa I and Bsm I were typed by PCR-RFLP among 162 patients and 167 healthy controls who had been sensitized to tuberculosis. The relationship among two polymorphisms and the susceptibility of PTB were analyzed, and cases were grouped according to the genotypes. Results The genotype of AA were in 6.2%,Aa in 44.5%,aa in 49.4% among patients while AA in 13.2%,Aa in 47.3%,aa in 39.5% among control group. Compared with control group, VDR genotype aa (mutant homozygotes) increased in the PTB patients (P<0.05,OR=1.493, 95% CI:0.964-2.312).The genotype of BB,Bb and bb were observed in 1.2%, 14.8% and 83.9% among tuberculosis group while in 0.6%, 11.9% and 87.4% among controls. The significant difference in these genotype frequencies was not observed between two groups(P>0.05,OR=1.009, 95% CI:0.560-1.816). Conclusion The VDR gene polymorphism of Apa Ⅰ might be associated with PTB in Tibetan Children at Qinghai-Tibetan Plateau and genotype aa might be a risk factor of it. But there was no evidence showed that Bsm Ⅰ polymorphism was associated with PTB in Tibetan Children at Qinghai-Tibetan Plateau. |
| keywords:Vitamin D receptor Qinghai-Tibetan plateau Tibetan children Pulmonary tuberculosis |
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