建立小鼠哮喘模型两种不同方法的比较
投稿时间:2016-11-28  修订日期:2016-12-20  点此下载全文
引用本文:袁丽粉,乔建瓯,王健.建立小鼠哮喘模型两种不同方法的比较[J].医学研究杂志,2017,46(8):132-134,138
DOI: 10.11969/j.issn.1673-548X.2017.08.032
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作者单位E-mail
袁丽粉 200011 上海交通大学医学院附属第九人民医院呼吸内科  
乔建瓯 200011 上海交通大学医学院附属第九人民医院呼吸内科  
王健 200011 上海交通大学医学院附属第九人民医院呼吸内科 wangjian0628@163.com 
基金项目:上海交通大学医学院科技基金资助项目(12XJ10020)
中文摘要:目的 比较两种小鼠哮喘模型建立方法的优缺点。方法 30只BALB/c小鼠随机正常对照组、OVA组、OVA/RSV组分为3组:采用卵白蛋白和氢氧化铝混悬液致敏,予以卵白蛋白持续雾化以及RSV多次滴鼻激发分别建立OVA组及OVA/RSV组哮喘小鼠模型,对照组采用无菌注射用水致敏和雾化激发。末次激发24h后,测定肺功能,行支气管肺泡灌洗液中细胞分类计数,HE染色观察其肺组织病理改变。结果 各模型组与对照组相比较,均有明显的哮喘症状,病理学结果显示气道壁增厚明显,管腔可见狭窄,管壁及其周围大量炎性细胞浸润,而且OVA/RSV组小鼠肺组织病理损害较OVA组明显加重。增强呼气间歇(Penh)值OVA/RSV组小鼠较OVA组小鼠明显升高(P<0.05),其支气管肺泡灌洗液中中性粒细胞计数、单核细胞、淋巴细胞及嗜酸性粒细胞计数显著高于OVA组(P<0.05)。结论 通过OVA致敏并予以RSV诱导建立哮喘小鼠模型,是一种更好地模拟人类哮喘发病机制的建模方法。
中文关键词:哮喘  卵白蛋白  RSV  动物模型
 
Comparison of Two Different Methods of Establishment of Mouse Asthma Model
Abstract:Objective To compare the advantages and disadvantages of the two methods. Methods Thirty BALB/c mice were randomly divided into 3 groups:normal control group, OVA group and OVA/RSV group. With ovalbumin and hydroxide suspension sensitized, OVA mouse asthma model group and OVA/RSV group were established respectively by continuous atomization of ovalbumin and repeated nasal drops of RSV. The control group was established by sensitization and atomization excitation of sterile injection water. 24 hours after the last stimulation, the lung function was measured. The cell sorting and counting in bronchoalveolar lavage fluid were performed. The pathological changes of lung tissue were observed by HE staining. Results Compared with the control group, the model groups have obvious symptoms of asthma, airway wall thickening, lumen stenosis and a large number of inflammatory cell infiltration. And pathological damage of lung tissue in OVA/RSV group was significantly increased, compared with OVA group. The enhanced expiratory pause (Penh) values of OVA/RSV group were significantly higher than those in group OVA (P<0.05), and the number of neutrophils, monocytes, lymphocytes and eosinophils in BALF were significantly higher than those in OVA group (P<0.05). Conclusion The establishment of asthma mouse model by OVA sensitization and RSV induction is a better way to simulate the human asthma pathogenesis.
keywords:Asthma  Ovalbumin  RSV  Animal model
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