九节龙皂苷对胃肠平滑肌功能的兴奋作用
投稿时间:2016-12-28  修订日期:2017-01-14  点此下载全文
引用本文:徐志立,陶小军,张明波,窦德强,康廷国.九节龙皂苷对胃肠平滑肌功能的兴奋作用[J].医学研究杂志,2017,46(9):42-45
DOI: 10.11969/j.issn.1673-548X.2017.09.012
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作者单位E-mail
徐志立 116600 大连, 辽宁中医药大学药学院  
陶小军 116600 大连, 辽宁中医药大学药学院  
张明波 116600 大连, 辽宁中医药大学药学院  
窦德强 116600 大连, 辽宁中医药大学药学院  
康廷国 116600 大连, 辽宁中医药大学药学院 kangtg@lnutcm.edu.cn 
基金项目:中国博士后科学基金资助项目(2012M510834)
中文摘要:目的 考察九节龙皂苷对大鼠及小鼠不同状态下胃肠功能的兴奋作用。方法 给予正常小鼠及阿托品导致胃肠功能障碍小鼠九节龙皂苷后测定其胃排空及肠推进,分别测定在正常克氏液和低钙克氏液中孵育的大鼠离体小肠收缩性,并用Western blot法检测九节龙皂苷对便秘大鼠肠肌球蛋白轻链激酶(MLCK)表达的影响。结果 九节龙皂苷可促进正常及胃肠功能障碍小鼠的胃排空及肠推进,增强大鼠离体小肠收缩性,且可刺激便秘大鼠MLCK的表达。结论 九节龙皂苷可促进平滑肌MLCK的表达,进而增强胃肠平滑肌运动功能。
中文关键词:九节龙皂苷  胃肠功能  肌球蛋白轻链激酶
 
Stimulating Effects of Ardipusilloside-Ⅰ on Gastro-intestinal Function
Abstract:Objective To investigate the stimulating effects of ardipusilloside-1 (ARD-Ⅰ) on gastro-intestinal function. Methods The gastric emptying and intestinal propulsion in normal mice or gastro-intestinal dysfunction mice induced by atropine were determined respectively, the contractibility of isolated intestine in rats was researched, and the expression of myosin light chain kinase (MLCK) in intestine in rats was detected by Western blot method. Results ARD-Ⅰ could promote the gastric emptying and intestinal propulsion in mice in any state, improve the contractibility of isolated intestine in rats incubated in normal krebs solution or low calcium krebs solution, and enhance the expression of MLCK in intestine in rats. Conclusion ARD-Ⅰ showed stimulating effects obviously on gastro-intestinal function, which might be reached by increasing the expression of smooth muscle MLCK.
keywords:Ardipusilloside-Ⅰ (ARD-Ⅰ)  Gastro-intestinal function  Myosin light chain kinase (MLCK)
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