| 慢病毒介导lncRNA-AK058003过表达对肝癌裸鼠移植瘤生长的影响 |
| 投稿时间：2016-12-02 修订日期：2016-12-28 点此下载全文 |
| 引用本文：周宇杰,徐细明,梁慧玲.慢病毒介导lncRNA-AK058003过表达对肝癌裸鼠移植瘤生长的影响[J].医学研究杂志,2017,46(9):46-50 |
| DOI：
10.11969/j.issn.1673-548X.2017.09.013 |
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| 基金项目:湖北省自然科学基金资助项目（2012FKC14301） |
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| 中文摘要:目的 探究上调SK-Hep1肝癌细胞中lncRNA-AK058003的表达对肝癌裸鼠移植瘤生长的影响。方法 采用慢病毒转染的方法将lncRNA-AK058003的重组质粒和空质粒分别转入SK-Hep1肝癌细胞株中，经新霉素筛选出lncRNA-AK058003稳定过表达的细胞株，用定量PCR的方法检测lncRNA-AK058003在SK-Hep1肝癌细胞中的过表达，并且将稳定过表达lncRNA-AK058003的SK-Hep1肝癌细胞和含有空质粒的SK-Hep1肝癌细胞分别注射入裸鼠腋下皮下，定期测量裸鼠移植瘤的长径和短径，从而计算出移植瘤的体积，饲养35天后测量移植瘤的重量。结果 构建稳定过表达lncRNA-AK058003的肝癌细胞株，裸鼠皮下荷瘤实验结果显示，过表达组裸鼠移植瘤的体积（308.4±439.4mm3）、重量（0.464±0.518g）和生长速度均低于空质粒组（1410.0±973.0mm3 ，1.363±0.856g，P < 0.05）。结论 lncRNA-AK058003对肝癌裸鼠移植瘤的增殖具有抑制作用。 |
| 中文关键词:lncRNA-AK058003 肝癌 裸鼠移植瘤 |
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| Effects of lncRNA-AK058003 Overexpression on the Growth of Human Hepatocellular Carcinoma in Nude Mice |
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| Abstract:Objective To investigate up-regulation of lncRNA-AK058003 expression in SK-Hep1 hepatocellular carcinoma cells and its effect on the growth of human hepatocellular carcinoma xenograft tumor in nude mice. Methods The recombinant plasmid of lncRNA-AK058003 and empty plasmid were transferred into SK-Hep1 human hepatocellular carcinoma cell line by the method of slow virus transfection. The overexpression of lncRNA-AK058003 cell line was screened by neomycin. Overexpression of lncRNA-AK058003 in SK-Hep1 hepatocarcinoma cells was detected by quantitative PCR. SK-Hep1 hepatocellular carcinoma cells stably overexpressing lncRNA-AK058003 and SK-Hep1 hepatocellular carcinoma cells containing empty plasmids were injected subcutaneously into armpit of nude mice. The long and short diameters of the transplanted tumor were measured regularly to calculate the volume of the xenograft tumor. After 35 days of feeding we measure the weight of the xenograft tumor. Results The recombinant plasmid of lncRNA-AK058003 was successfully constructed and stably over expressed in hepatocellular carcinoma cells. The experimental results of subcutaneous tumor bearing nude mice showed that the volume(308.4±439.4mm3), weight (0.464±0.518g)and growth rate of the xenograft tumor in the over expression group were lower than that of the empty plasmid group(1410.0±973.0mm3 ,1.363±0.856g,P < 0.05). Conclusion LncRNA-AK058003 has inhibitory effect on the proliferation of human hepatocellular carcinoma xenograft tumor in nude mice. |
| keywords:LncRNA-AK058003 Liver cancer Xenograft tumor in nude mice |
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