| 七氟烷预处理对大鼠肢体缺血/再灌注所致肺损伤的影响 |
| 投稿时间：2016-06-25 修订日期：2016-07-13 点此下载全文 |
| 引用本文：何峰,杜耀武.七氟烷预处理对大鼠肢体缺血/再灌注所致肺损伤的影响[J].医学研究杂志,2017,46(9):126-130 |
| DOI：
10.11969/j.issn.1673-548X.2017.09.033 |
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| 中文摘要:目的 评价七氟烷（Sevo）预处理对大鼠肢体缺血/再灌注（IR）所致肺损伤的影响。方法 健康成年清洁级Sprague-Dawley （SD）大鼠45只，采用随机数字表法分为3组（n=15）：假手术组（Sham组）大鼠只分离股静脉和股动脉，但不夹闭；肢体缺血/再灌注（IR）组大鼠夹闭股动脉缺血3h，再灌注3h；Sevo预处理+IR组（SIR组）吸入2.5%七氟烷30min，15min后制备肢体IR模型。实验结束后留取左肺，测定肺组织湿干/重比（W/D）和总肺水含量（TLW），光镜下观察肺组织病理学改变并测定肺组织损伤定量评估（IQA），电镜下观察肺组织超微结构改变，反转录-PCR （RT-PCR）和蛋白免疫印迹法（Western blot法）分别检测肺组织葡萄糖调节蛋白78（GRP78）和CCAAT增强子结合蛋白（C/EBP）同源蛋白（CHOP）的表达水平。结果 与Sham组比较，IR组大鼠肺组织W/D、TLW和IQA均升高（P<0.05）。SIR组大鼠肺组织W/D、TLW和IQA均较IR组降低（P<0.05）。IR组大鼠肺组织形态学结构和超微结构发生明显损伤，而SIR组大鼠肺组织形态学结构和超微结构损伤均明显减轻。与Sham组比较，IR组大鼠肺组织GRP78和CHOP mRNA及蛋白表达水平均升高（P<0.05）。而SIR组大鼠肺组织GRP78和CHOP mRNA及蛋白表达水平均较IR组降低（P<0.05）。结论 七氟烷预处理对肢体IR所致肺损伤发生的大鼠肺脏具有较好的保护作用，其机制可能与其抑制肺组织中过度的未折叠蛋白反应（UPR）有关。 |
| 中文关键词:七氟烷 预处理 肺损伤 缺血/再灌注 肢体 |
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| Effect of Sevoflurane Preconditioning on Lung Injury Induced by Ischemia/reperfusion of Hind Limbs in Rats |
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| Abstract:Objective To investigate the effect of sevoflurane (Sevo) preconditioning on lung injury induced by ischemia/reperfusion (IR) of hind limbs in rats. Methods Forty-five healthy adult Sprague-Dawley (SD) rats of clean grade were randomly allocated into three groups, 15 mice per group. Femoral veins and femoral arteries of rats were isolated but not clipped in Sham operation group (Sham group). Femoral arteries of rats were clipped for ischemia for 3h, followed reperfusion for 3h in ischemia/reperfusion group (IR group). Rats were inhaled with 2.5% sevoflurane for 30 min, followed establishing the model of IR of hind limbs after 15min in Sevo preconditioning + IR group (SIR group). Rats were euthanized after experimental time out, and left lung tissue was excised. Wet lung weight to dry lung weight (W/D) and total lung water content (TLW) were tested. Pathological changes of lung tissue were observed and index of quantitative evaluation for alveolar damage (IQA) was tested by light microscope, and changes of ultrastructure of lung tissue were observed by transmission electron microscope. The expressions of glucose-regulated protein 78 (GRP78) and CCAAT enhancer binding protein (C/EBP) homologous proteins (CHOP) protein were detected by reverse transcription PCR (RT-PCR) and Western Blot, respectively. Results Compared to Sham group, W/D, TLW and IQA were all notably higher (P<0.05) in IR group. W/D, TLW and IQA of SIR group were all lower (P<0.05) than those of IR group. The injury of morphological structure and ultrastructure of lung tissue was distinct in IR group but alleviated in SIR group. Compared to Sham group, the expression of GRP78 and CHOP was higher (P<0.05) in IR group. The expression of GRP78 and CHOP in SIR group was lower (P<0.05) than that in IR group. Conclusion Sevo preconditioning has protective effect on rats with lung injury induced by IR of hind limbs, which may be related to inhibition of excessive unfolded protein response (UPR). |
| keywords:Sevoflurane Preconditioning Lung injury Ischemia/reperfusion Hind limbs |
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