| 黄芪甲苷对苯并芘诱导RAW264.7细胞MMPs表达的保护作用 |
| 投稿时间：2017-01-16 修订日期：2017-02-28 点此下载全文 |
| 引用本文：邢程,吴少泽,王娇妮,黄凯宇,季亢挺,薛杨静.黄芪甲苷对苯并芘诱导RAW264.7细胞MMPs表达的保护作用[J].医学研究杂志,2017,46(10):31-34 |
| DOI：
10.11969/j.issn.1673-548X.2017.10.009 |
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| 基金项目:国家自然科学基金资助项目（81573185）；浙江省省科技厅基金资助项目（2014C33163）；温州市科技局基金资助项目（Y20140678） |
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| 中文摘要:目的 观察黄芪甲苷（astragaloside IV，ASIV）对苯并芘（benzopyrene，Bap）诱导RAW264.7巨噬细胞MMPs表达的保护作用，探讨腹主动脉瘤防治的可能新策略。方法 应用Western blot法观察Bap对RAW264.7巨噬细胞基质金属蛋白酶-9/12（MMP-9、MMP-12）、NF-κB、TNF-α等表达的变化及黄芪甲苷的作用，利用流式细胞仪观察黄芪甲苷对巨噬细胞ROS表达的影响。结果 Bap能够诱导RAW264.7巨噬细胞MMP-9、MMP-12等金属蛋白酶的表达升高和NF-κB、TNF-α等炎性指标的活化。黄芪甲苷具有降低RAW264.7巨噬细胞因Bap诱导而表达升高的MMP-9、MMP-12、NF-κB、TNF-α，同时具有清除ROS的作用。结论 黄芪甲苷能够降低Bap刺激RAW264.7巨噬细胞引起的MMP-9、MMP-12、NF-κB、TNF-α的表达升高，并具有清除ROS的作用。 |
| 中文关键词:黄芪甲苷 RAW264.7巨噬细胞 苯并芘 基质金属蛋白酶 |
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| Benzopyrene Induced the Expression of MMPs and the Effects of Astragaloside in RAW264.7 Cells |
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| Abstract:Objective To explore the effect of astragaloside IV (ASIV) in Bap induced the expression of MMPs on RAW264.7 cells, to provide a new control strategy of abdominal aortic aneurysm caused by smoking. Methods We observed the influence of ASIV on matrix metalloproteinase-9/12 (MMP-9,MMP-12) and NF-κB,TNF-α of RAW264.7 cells in vitro by western blotting. We used flow cytometry instrument to analysis whether ASIV has the role of reducing ROS. Results Bap caused expression of MMP-9, MMP-12, NF-κB, TNF-α increased in cultured RAW264.7 cells. ASIV has the effect of decreasing the expression of MMP-9, MMP-12, NF-κB, TNF-α, otherwise it can eliminate the ROS which was caused by Bap incultured RAW264.7 cells. Conclusion ASIV can decrease the expression of MMP-9,MMP-12, NF-κB,TNF-α and eliminate the ROS in RAW264.7 cells cultured with Bap. |
| keywords:Astragaloside RAW264.7 cells Benzopyrene Matrix metalloproteinases |
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