| 蓝莓花青素对由H2O2诱导人脐静脉内皮细胞氧化应激损伤的保护作用 |
| 投稿时间：2016-12-21 修订日期：2016-12-21 点此下载全文 |
| 引用本文：赵凯,郑源强,贾宇臣.蓝莓花青素对由H2O2诱导人脐静脉内皮细胞氧化应激损伤的保护作用[J].医学研究杂志,2017,46(10):113-115 |
| DOI：
10.11969/j.issn.1673-548X.2017.10.029 |
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| 基金项目:内蒙古自治区科技厅科技计划项目（20120402）；内蒙古自治区研究生科研创新基金资助项目（B20161012615） |
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| 中文摘要:目的 通过建立人脐静脉内皮细胞株（human umbilical vein endothelial cells，HUVECs）氧化应激损伤细胞模型，探讨蓝莓花青素是否能够对H2O2引起的细胞损伤具有保护作用。方法 采用过氧化氢（H2O2）创建ECV304氧化应激损伤细胞模型，实验分为正常对照组、H2O2组、蓝莓花青素低、中、高剂药物组，药物组中加入蓝莓花青素培养2h后，再加入H2O2继续培养6h，采用MTT法检测细胞活力，流式细胞术检测细胞周期变化情况及细胞凋亡率。结果 细胞活力与H2O2浓度及其作用的时间呈相关性下降趋势，在一定浓度范围内蓝莓花青素对细胞具有保护作用。结论 蓝莓花青素对由H2O2引起的氧化应激损伤具有一定保护作用。 |
| 中文关键词:蓝莓花青素 氧化应激损伤 人脐静脉内皮细胞株 |
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| Protective Effect of Blueberry Anthocyanins on Oxidative Stress Injury by H2O2 of Human Umbilical Vein Endothelial Cells |
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| Abstract:Objective To study the protective effect of blueberry anthocyanins on H2O2 induced human umbilical vein endothelial cells,HUVECs. Methods The cultured cells were divided into 3 groups:normal control group, H2O2 model group, blueberry anthocyanins group. MTT method was used to observe cell viability. Annexin V-FITC/PI straining was used to observe cell apoptosis. Flow cytometry was used to detect cell cycle. Results H2O2 could induce HUVECs oxidative stress injury. Cell survival rate was positive correlated with drug concentration and action time. In the certain concentration range blueberry anthocyanin had a protective effect. Conclusion Blueberry anthocyanin can protect H2O2 induced oxidative stress injury in HUVECs. |
| keywords:Blueberry anthocyanins Oxidative stress injury Human umbilical vein endothelial cells |
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