| 生长激素释放肽-2对自发性高血压大鼠心肌纤维化的抑制作用 |
| 投稿时间：2017-02-01 修订日期：2017-02-09 点此下载全文 |
| 引用本文：张鹏,朱元杰,闫莉,谷靖丽,曹济民.生长激素释放肽-2对自发性高血压大鼠心肌纤维化的抑制作用[J].医学研究杂志,2017,46(11):16-19 |
| DOI：
10.11969/j.issn.1673-548X.2017.11.006 |
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| 基金项目:国家自然科学基金资助项目（81670313，81300139） |
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| 中文摘要:目的 研究生长激素释放肽-2（GHRP-2）是否可抑制心肌纤维化。方法 由于自发性高血压大鼠（SHR）存在自然发生的严重心肌纤维化，本研究利用SHR作为心肌纤维化模型。SHR从12周龄开始给予GHRP-2皮下注射治疗（100μg/kg，每日1次，共4周），同龄Wistar大鼠皮下注射等量生理盐水作为正常对照组；利用苦味酸天狼猩红染色法显示SHR左心室肌Ⅰ型胶原和Ⅲ型胶原，用Van Gienson染色法显示左心室总胶原水平；用羟脯氨酸含量测定法评估心肌中胶原合成水平。结果 与正常对照组相比，未经处理的SHR左心室肌中Ⅰ型和Ⅲ型胶原明显增多，羟脯氨酸含量也显著增高。GHRP-2治疗可明显降低SHR心肌中Ⅰ型、Ⅲ型胶原和总胶原的水平，并降低羟脯氨酸含量。结论 GHRP-2可以通过抑制胶原合成而减轻SHR左心室纤维化。 |
| 中文关键词:生长激素释放肽 心肌纤维化 自发性高血压大鼠 胶原 |
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| GHRP-2 Attenuates Cardiac Fibrosis in Spontaneously Hypertensive Rats |
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| Abstract:Objective To investigate whether growth hormone releasing peptide-2 (GHRP-2) could attenuate cardiac fibrosis in spontaneously hypertensive rats (SHR).Methods SHR shows severe cardiac fibrosis and were used as a model of cardiac fibrosis in this study. SHR were treated with GHRP-2 (100μg/kg, sc, qd) for 4 weeks starting from the 12th week of birth. Picro-sirius red staining was used to visualize type Ⅰ and type Ⅲ collagens, Van Gienson staining was applied to visualize total collagens, and hydroxyproline assay was used to quantitatively evaluate the synthetic level of myocardial collagen.Results Compared with control Wistar rats, GHRP-2 treatment significantly decreased the levels of type Ⅰ and type Ⅲ collagens and the total collagens, and also reduced the level of hydroxyproline.Conclusion GHRP-2 attenuates cardiac fibrosis via suppressing cardiac synthesis of collagens. |
| keywords:Growth hormone releasing peptides Cardiac fibrosis Spontaneously hypertensive rats Collagen |
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