| 槲皮素抑制ADMA引起的脐静脉内皮细胞内质网应激和细胞凋亡 |
| 投稿时间：2017-03-02 修订日期：2017-03-21 点此下载全文 |
| 引用本文：郭维康,刘英杰,刘文虎.槲皮素抑制ADMA引起的脐静脉内皮细胞内质网应激和细胞凋亡[J].医学研究杂志,2017,46(11):34-37 |
| DOI：
10.11969/j.issn.1673-548X.2017.11.010 |
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| 基金项目:国家自然科学基金资助项目（81570660）；北京市优秀人才培养基金资助项目（2016000021469G223） |
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| 中文摘要:目的 探讨槲皮素改善ADMA引起脐静脉内皮细胞凋亡的可能机制。方法 体外培养人脐静脉内皮细胞，应用流式细胞仪技术检测细胞凋亡情况，应用Western blot法检测内质网应激蛋白PERK磷酸化水平及IRE1的蛋白表达量，用RT-PCR检测ATF4及CHOP mRNA表达量。结果 ADMA诱导脐静脉内皮细胞凋亡，槲皮素可以抑制ADMA引起的PERK磷酸化及IRE1蛋白表达，并可以减少ATF4及CHOP mRNA量，继而改善ADMA引起的细胞凋亡。结论 槲皮素抑制ADMA引起的脐静脉内皮细胞内质网应激继而抑制细胞凋亡。 |
| 中文关键词:ADMA 内质网应激 细胞凋亡 槲皮素 |
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| Quercetin Inhibited ADMA-induced Endoplasmic Reticulum Stress Responses and HUVECs Apotosis |
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| Abstract:Objective To study the effect of quercetin on ADMA induced endoplasmic reticulum stress responses (ER stress).Methods Human umbilical veins endothelial cells (HUVECs) were cultured, and left untreated or challenged for 24h with 100μmol/L ADMA in the absence and presence of 20μmol/L quercetin, the PERK and IRE1 was determined by Western blot. ATF4 and CHOP mRNA was detected by RT-PCR. Cells apoptosis was detected by flow cytometry.Results ADMA induced HUVECs apoptosis. Quercetin inhibited ADMA-induced PERK and IRE1 protein expression and ATF4 and CHOP mRNA expression. At last, Quercetin inhibited ADMA-induced cells apoptosis.Conclusion ADMA induced ER stress and HUVECs apotosis while quercetin inhibited ADMA-induced ER stress and apoptosis. |
| keywords:ADMA ER stress Apoptosis Quercetin |
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