| JNK信号通路在白介素-1β介导人胚肺成纤维细胞活化中的作用 |
| 投稿时间：2016-12-24 修订日期：2017-01-11 点此下载全文 |
| 引用本文：张慧,林蓓蓓,张维溪,林立,李昌崇.JNK信号通路在白介素-1β介导人胚肺成纤维细胞活化中的作用[J].医学研究杂志,2017,46(11):44-48 |
| DOI：
10.11969/j.issn.1673-548X.2017.11.012 |
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| 基金项目:国家自然科学基金资助项目（30973232） |
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| 中文摘要:目的 探讨c-Jun氨基末端激酶（JNK）信号转导通路在白细胞介素-1β（IL-1β）介导的人胚肺成纤维细胞（FB）活化中的作用。方法 体外培养人胚肺FB，用IL-1β、JNK抑制剂（SP600125）作用于细胞，采用免疫蛋白印迹法（Western blot）检测细胞JNK/SAPK磷酸化水平和α-平滑肌激动蛋白（α-SMA）的表达，RT-PCR方法检测纤溶酶原激活物抑制剂（PAI-1）、纤维连接蛋白（FN）、神经生长因子（NGF）和α-SMA mRNA的相对表达量，ELISA法检测培养上清液中NGF及白细胞介素-6（IL-6）水平。结果 在IL-1β刺激下，人胚肺FB中α-SMA及磷酸化JNK蛋白表达显著高于对照组，同时PAI-1、FN、NGF和α-SMA mRNA的表达及培养上清液中NGF、IL-6水平均明显高于对照组（P<0.05）；SP600125显著抑制IL-1β诱导的上述指标表达增加（P<0.05）。结论 IL-1β促进人胚肺FB活化及细胞外基质聚集，JNK信号转导通路参与这一过程。 |
| 中文关键词:白细胞介素-1β JNK信号通路 人胚肺成纤维细胞 细胞外基质 |
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| Role of c-Jun N-terminal Kinase Signal Pathway on Interleukin-1β Induced Activation of Human Embryonic Lung Fibroblast |
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| Abstract:Objective To explore the role of c-Jun N-terminal kinase (JNK) signal pathway on interleukin-1β (IL-1β) induced activation of human embryonic lung fibroblasts (FB).Methods The human embryonic lung fibroblasts were cultured in vitro and treated with IL-βor JNK inhibitor SP600125. The expression of phosphorylated JNK (p-JNK) and α-smooth muscle actin (α-SMA) were examined by Western blot, and the plasminogen activator inhibitor of endothelial cell type (PAI-1), fibrinogen (FN), nerve growth factor (NGF) and α-SMA mRNA were detected by RT-PCR. In addition, the concentrations of NGF and interleukin-6 (IL-6) levels in the culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA).Results We found that under the treatment of IL-1β, the JNK signal pathway in human lung fibroblast was activated. The expression of p-JNK increased significantly compared with the control group (P<0.05). mRNA expressions of PAI-1, FN,NGF, α-SMA, and the concentrations of NGF and IL-6 in the culture supernatant were all increased in the IL-1β treated groups (P<0.05). However, the SP600125 reversed the effect of IL-1β on the expression of p-JNK, α-SMA, FN, NGF, and IL-6 mentioned above.Conclusion These findings provided evidence that JNK signal pathway may play an important role in the IL-1β induced human lung fibroblast activation and extracellular matrix synthesis. |
| keywords:Interleukin-1β(IL-1β) JNK Human embryonic lung fibroblast Extracellular matrix |
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