| 绝经期和生育期雌激素缺乏小鼠细胞因子模式的研究 |
| 投稿时间：2017-01-15 修订日期：2017-02-27 点此下载全文 |
| 引用本文：邵明君,胡旻,何云芹,朱以军,唐飞.绝经期和生育期雌激素缺乏小鼠细胞因子模式的研究[J].医学研究杂志,2017,46(11):80-83 |
| DOI：
10.11969/j.issn.1673-548X.2017.11.020 |
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| 基金项目:浙江省自然科学基金资助项目（Y13H040019） |
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| 中文摘要:目的 观察雌激素（E2）缺乏状态下年轻与老龄化C57BL/6N小鼠脾脏IL-2、6、10和TNF-α水平的变化。方法 清洁级健康雌性C57BL/6小鼠80只，分为手术去卵巢组、药物去卵巢组、自然绝经组及发情间期组（对照组），经Con-A刺激后RT-PCR技术检测4组小鼠脾脏IL-2、6、10和TNF-α水平，ELISA检测血清E2水平证实分组可靠性。结果 3组实验组血清E2水平均明显低于对照组（P<0.01），而E2水平在3组实验组内比较差异无统计学意义。小鼠脾脏表达IL-2、IL-6和TNF-α水平在自然绝经组升高，与其他3组比较差异均有统计学意义（P<0.05）；而3组实验组IL-10水平略低于对照组，差异无统计学意义（P>0.05）；然而，年轻组别中（发情间期组、手术去卵巢组、药物去卵巢组）未发现有IL-2、IL-6、IL-10和TNF-α mRNA表达的差异。结论 Con-A刺激后E2缺乏状态下年轻与老龄化C57BL/6N小鼠之间细胞因子模式是不同的，E2缺乏对IL-6、IL-2和TNF-α水平影响作用有限。 |
| 中文关键词:手术去卵巢 药物去卵巢 绝经 细胞因子 |
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| Different Cytokines Patten in Young and Aged Mice with Estradiol Deficiency |
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| Abstract:Objective To compare the levels of IL-2, IL-6,IL-10 and TNF-α between young and aged C57BL/6N mice with the common feature of low E2 status.Methods Twenty natural aged, 20 surgical variectomy and 20 medical variectomy of C57BL/6N mice were chosen in our studies. 20 stage of diestrus served as controls. Blood and spleens were collected 4 hours after injection of con-A. Reverse transcription-pelymerase chain reaction (RT-PCR) was used to determine the mRNA levels of IL-2, IL-6, IL-10 and TNF-α in spleen cells. Serum E2 levels were assessed using enzyme linked immunosorbent assay (ELISA).Results The serum E2 level of three experiment groups was significantly lower than that of control group (P<0.01), while the difference was not significant when compared it within experiment groups. The levels of IL-2, IL-6 and TNF-α were increased in aged group, and the difference reached statistically significant when compared them with any other groups(P<0.05). While the IL-10 level was slight decreased in three experiment groups compared with control group (P>0.05).Conclusion The cytokines response to Con-A stimulus between young and aged C57BL/6N mice with estradiol deficiency was different. The effect of E2 to these cytokines may be marginal. |
| keywords:Surgical variectomy Medical variectomy Aged mice Cytokines |
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