| 红参水提物对哮喘气道炎症小鼠的治疗作用及其机制研究 |
| 投稿时间：2017-02-17 修订日期：2017-03-17 点此下载全文 |
| 引用本文：黄丽,靳庆霞.红参水提物对哮喘气道炎症小鼠的治疗作用及其机制研究[J].医学研究杂志,2017,46(11):161-164 |
| DOI：
10.11969/j.issn.1673-548X.2017.11.039 |
| 摘要点击次数: 943 |
| 全文下载次数: 661 |
|
|
| 中文摘要:目的 探讨红参水提物对哮喘气道炎症小鼠模型的治疗作用及其机制。方法 选取BABL/c小鼠75只，采用随机数字表法分为正常组（等量生理盐水）、模型对照组（等量生理盐水）、低剂量组（红参水提物100mg/kg）、高剂量组（红参水提物200mg/kg）、阳性对照组（地塞米松0.5mg/kg）各15只，除正常组外均成功制作哮喘气道炎症小鼠模型，给予对应的处理措施，对比各组小鼠末次激发后24h的肺泡灌洗液（BALF）中的各种炎性细胞因子、血清免疫球蛋白E （IgE）、肺组织中羟脯氨酸含量，并采用HE染色观察各组大鼠肺组织病理变化。结果 模型对照组BALF中白细胞介素-4（IL-4）、IL-5、IL-14、转化生长因子β1（TGF-β1）、血管内皮生长因子（VEGF）、血清免疫球蛋白E （IgE）、肺组织羟脯氨酸均高于正常组（P<0.01）；低剂量组、高剂量组及阳性对照组的BALF中IL-4、IL-5、IL-14、TGF-β1、VEGF水平、血清IgE、肺组织羟脯氨酸均低于模型对照组（P<0.01）；高剂量组BALF中IL-4、IL-5、IL-14、TGF-β1、VEGF水平、血清IgE、肺组织羟脯氨酸均低于低剂量组（P<0.05）。结论 红参水提物主要通过减轻哮喘小鼠模型的气道炎性反应程度达到治疗作用。 |
| 中文关键词:红参 哮喘 气道炎症 机制 |
| |
| Experimental Study on the Effect and Mechanism of Water Extract of Red Ginseng on Asthmatic Mice with Airway Inflammation |
|
|
| Abstract:Objective To investigate the effect and mechanism of water extract of red ginseng on asthmatic mice with airway inflammation.Methods Totally 75 BALB/c mice were selected, they were divided into normal group (Physiological saline equivalent), model control group (Physiological saline equivalent), low dose group (Water extract of red ginseng, 100mg/kg), high dose group (Water extract of red ginseng, 100mg/kg) and positive control group (Dexamethasone, 0.5mg/kg) by random number table method. There were 15 mice in each group. In addition to the normal group, all the other groups successfully produced the asthmatic airway inflammation model in mice, and the corresponding treatment measures were given. Comparison of mice after the last challenge 24h in bronchoalveolar lavage fluid (BALF) of various inflammatory cytokines and serum immunoglobulin E (IgE), Hydroxyproline content in lung tissue and pathological changes were observed in lung tissue of rats with HE.Results The model control group BALF interleukin interleukin-4(IL-4), interleukin-5(IL-5), interleukin-14(IL-14), transforming growth factor-β1(TGF-β1), Vascular endothelial growth factor(VEGF), serum immunoglobulin E(IgE), hydroxyproline content in lung tissue were higher than the normal group (P<0.01).The levels of IL-4, IL-5, IL-14, TGF-β1, VEGF, IgE and Hydroxyproline content in lung tissue in the low dose group, the high dose group and the positive control group were lower than that in the model control group (P<0.01) in BALF. The levels of IL-4, IL-5, IL-14, TGF-β1, VEGF, IgE and hydroxyproline content in lung tissue in the high dose group of BALF were lower than those in the low dose group (P<0.05).Conclusion The water extract of red ginseng can achieve the therapeutic effect by reducing the airway inflammatory reaction of the asthmatic mice model. |
| keywords:Red ginseng Asthma Airway inflammation Mechanism |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
