| 斯钙素2对乳腺癌细胞侵袭、转移的影响及其机制研究 |
| 投稿时间：2017-03-02 修订日期：2017-04-20 点此下载全文 |
| 引用本文：郭昭泽,谢国柱,李亚,叶长生.斯钙素2对乳腺癌细胞侵袭、转移的影响及其机制研究[J].医学研究杂志,2017,46(12):63-67 |
| DOI：
10.11969/j.issn.1673-548X.2017.12.017 |
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| 基金项目:广东省科技计划自筹类项目（2016ZC0075） |
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| 中文摘要:目的 本研究探讨STC2对乳腺癌细胞侵袭转移的影响及其作用机制。方法 通过shRNA干扰降低231 HM细胞STC2表达观察纤维细胞形态，平板克隆实验统计分析克隆数；免疫共沉淀（CO-IP）进行检测相关蛋白的表达。结果 通过shRNA干扰降低231 HM细胞STC2表达之后，231 HM呈现出高转移特性的纤维细胞形态，同时细胞的侵袭转移能力增加；相反，在231细胞过表达STC2后细胞的侵袭、转移能力减弱。此外，降低231 HM细胞的STC2表达之后，与对照相比其抵抗射线诱导的凋亡能力增强，而过表达STC2后的231细胞抗射线诱导凋亡的能力降低。结论 STC2可能通过调节乳腺癌中PKC/Claudin-1通路进而调节EMT过程最终影响乳腺癌细胞的侵袭、转移能力。 |
| 中文关键词:乳腺癌 斯钙素2 侵袭转移 上皮间质转化 蛋白激酶C 紧密连接蛋白1 |
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| STC2 Suppresses Breast Cancer Cell Migration and Invasion Via the PKC-Claudin-1-mediated Signaling |
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| Abstract:Objective To study the effect of STC2 on invasion and metastasis of breast cancer cells and its mechanism.Methods By 231 HM shRNA interference cell STC2, we observed fiber cells expressing form, number plate cloning experiment statistical analysis.Immune co-precipitation (CO-IP) for testing related protein expression.Results By 231 HM STC2 cells expressing shRNA interference, 231 HM presents high transfer characteristics of fiber cell morphology, cell invasion and metastasis ability of increase at the same time;On the contrary, after 231 cells expressing STC2, cell invasion and metastasis ability induced.In addition, after reducing STC2 expression of 231 HM cells,its resistance to radiation-inducled apoptosis and expression of STC2 after 231 reduced, ability to resist radiation induced apoptosis of cells induled too.Conclusion Collectively, these results indicate that STC2 may inhibit EMT at least partially through the PKC/Claudin-1-mediated signaling in human breast cancer cells. |
| keywords:Breast cancer Stanniocalcin 2 Migration and invasion EMT PKC Claudin-1 |
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