鸦胆子素D对结肠癌HT29细胞株增殖的抑制及诱导其凋亡的机制
投稿时间:2016-12-26  修订日期:2017-04-04  点此下载全文
引用本文:郑艳艳,陈晶晶,王兆洪.鸦胆子素D对结肠癌HT29细胞株增殖的抑制及诱导其凋亡的机制[J].医学研究杂志,2017,46(12):85-89
DOI: 10.11969/j.issn.1673-548X.2017.12.022
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作者单位E-mail
郑艳艳 325000 温州市中西医结合医院肛肠外科、温州医科大学附属第二医院肝胆外科  
陈晶晶 325000 温州市中西医结合医院肛肠外科、温州医科大学附属第二医院肝胆外科  
王兆洪 325000 温州市中西医结合医院肛肠外科、温州医科大学附属第二医院肝胆外科 wangzhaohong55@163.com 
基金项目:温州市科技局计划项目(Y20160434)
中文摘要:目的 探讨鸦胆子素D对结肠癌细胞株HT29增殖的抑制及诱导其凋亡的机制。方法 人结肠癌细胞株HT29经不同浓度鸦胆子素分别作用24、48、72h后,应用CCK-8(Cell Counting Kit-8)法检测鸦胆子素D对HT29细胞的增殖抑制作用;流式细胞术检测鸦胆子素D对HT29凋亡的影响;Western blot法检测HT29细胞株经不同浓度鸦胆子素D处理24h后以及20μmol/L的鸦胆子素D处理24、48、72h后,细胞中JNK、p-JNK的表达。结果 不同浓度鸦胆子素D分别作用结肠癌HT29细胞24、48、72h后,细胞存活率随药物浓度升高,细胞存活率降低,细胞的凋亡率增加。HT29细胞株经不同浓度鸦胆子素D处理24h后以及20μmol/L的鸦胆子素D处理24、48、72h后,JNK蛋白表达随鸦胆子素D作用时间延长而下调,p-JNK则相应上调。结论 不同浓度的鸦胆子素D随着浓度的增加及时间的延长,结肠癌HT29细胞的存活率降低、凋亡率增高,呈明显的作用-时间-药物浓度依赖性。
中文关键词:鸦胆子素D  结肠癌  增殖  凋亡  JNK  p-JNK
 
Brucein D Inhibits Proliferation and Induces Apoptosis in Colon Adenocarcinoma Cell Line HT29
Abstract:Objective To investigate that brucein D (BD) possesses potent anti-colon cancer activity by inhibiting proliferation and inducing apoptosis in colon adenocarcinoma cell.Methods After human colon cancer cell line PANC-1 was treated with BD (0, 5, 10, 20, 30, 80)μmol/L for 24,48,72h,the cell proliferation was detected by cell counting kit-8(CCK-8)assay. The apoptosis of colon cancer cells was detected using Annexin V-FITC/PI. The protein expressions of JNK and p-JNK were detected using Western blot.Results After human colon cancer cell line PANC-1 was treated with BD for 24,48,72h, the cell survival rates decreased lower and apoptosis rate increased in concentration-and time-dependent manners. AS the concentration of BD got higher or the time got longer, the protein expression of JNK was down-regulated and that of p-JNK up-regulated significantly. In vivo, with the prolonged administration, the mean volume of xenograft tumor grew slowly. The protein expression of JNK was down-regulated and that of p-JNK up-regulated significantly in colon xenograft tumors cells.Conclusion With the increase of time and BD's concentration, the survival rates of colon cancer HT29 cells could be lower. Instead, the apoptosis rate has increased in a concentration-time-dependent manners.
keywords:Brucein D  Colon cancer  Proliferation  Apoptosis  JNK  p-JNK
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