| GFP小鼠对三维体外着床模型的定位及验证 |
| 投稿时间：2017-04-24 修订日期：2017-04-27 点此下载全文 |
| 引用本文：叶天民,彭鼎佳,高晶,王琨,霍然,肖佳,杨树标.GFP小鼠对三维体外着床模型的定位及验证[J].医学研究杂志,2018,47(1):66-69,98 |
| DOI：
10.11969/j.issn.1673-548X.2018.01.017 |
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| 基金项目:广东省自然科学基金资助项目（2015A030310470）；深圳市卫生和计划生育委员会基金资助项目（201501042）；深圳市科学技术创新委员会基金资助项目（JCYJ20150331142757399） |
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| 中文摘要:目的 本研究将基于已经建立的三维小鼠体外胚胎着床模型，运用绿色荧光（GFP）小鼠胚胎替代ICR小鼠胚胎，验证三维着床模型的可行性。方法 将孕第4天GFP小鼠及ICR小鼠囊胚与ICR小鼠的内膜组织进行体外共培养。观察两组胚胎黏着率的差异。对GFP小鼠囊胚组通过荧光显微镜进行观察，共培养后进行组织学研究。结果 GFP小鼠囊胚及ICR小鼠囊胚与ICR小鼠子宫内膜组织共培养28h后的胚胎黏着率分别为46.6%及54.8%；两组黏着率之间差异无统计学意义（P=0.364）。GFP小鼠囊胚与ICR小鼠子宫内膜组织共培养28h及40h后均可在荧光显微镜下见到胚胎，组织学研究证实GFP小鼠囊胚可以黏着于ICR小鼠子宫内膜。结论 三维着床模型确实能够实现不同来源小鼠胚胎的黏附着床。运用GFP小鼠胚胎还发现了胚胎与子宫内膜组织之间存在着信息及分子联系。 |
| 中文关键词:GFP小鼠 胚胎着床 模型 |
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| Confirm The three-dimensional in Vitro Embryo Implantation Model with GFP Mouse |
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| Abstract:Objective To confirm the three-dimentional in vitro embryo implantation model, the ICR mouse blastocyst was replaced by green fluorescent protien (GFP) mouse blastocyst in the study. Methods In vitro GFP and ICR embryo implantation was performed on ICR endometrium cultured. The attachment rates were compared. The GFP mouse blastocyst were checked by fluorescent microscopy, the histological study then followed. Results There was no significant different attachment rate between the GFP mouse blastocyst group (46.6%) and the ICR mouse blastocyst group (54.8%). The GFP mouse blastocyst can be seen with fluorescent microscopy after 28 and 40 hours' co-culture. The GFP mouse blastocyst can attach to ICR mouse endometrium. Conclusion The embryo from different mouse strain can attach to the mouse endometrium in the in vitro embryo implantation model. There may be information and molecular cross-talk between the GFP mouse embryoes and ICR mouse endometrium in this study. |
| keywords:GFP mouse Embryo implantation Model |
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