| 虾青素对AngⅡ诱导的心脏成纤维细胞胶原蛋白合成的影响 |
| 投稿时间：2017-04-27 修订日期：2017-05-11 点此下载全文 |
| 引用本文：陈素琴,黎明江,吴限,张文艳.虾青素对AngⅡ诱导的心脏成纤维细胞胶原蛋白合成的影响[J].医学研究杂志,2018,47(2):36-40 |
| DOI：
10.11969/j.issn.1673-548X.2018.02.010 |
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| 基金项目:国家自然科学基金资助项目（81170085） |
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| 中文摘要:目的 探讨虾青素对血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）诱导的心脏成纤维细胞（cardiac fibroblasts，CFs）胶原蛋白合成的影响。方法 体外培养SD乳鼠的CFs，分为5组，分别为对照组、AngⅡ组、AngⅡ+虾青素低剂量组（20μmol/L）、AngⅡ+虾青素中剂量组（40μmol/L）、AngⅡ组+虾青素高剂量组（80μmol/L），干预24h后，采用Western blot法检测Ⅰ型胶原蛋白的含量，RT-PCR检测α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）的含量，活细胞计数试剂盒（CCK8）测定CFs增殖情况。结果 AngⅡ可以使Ⅰ型胶原蛋白和α-平滑肌肌动蛋白表达上升（P<0.05），CFs增殖增加，不同浓度的虾青素均可以抑制这种作用（P<0.05）。结论 在一定的浓度范围内，虾青素可抑制心脏成纤维细胞胶原蛋白的合成，抑制心脏成纤维细胞的增殖。 |
| 中文关键词:虾青素 心脏成纤维细胞 心肌纤维化 Ⅰ型胶原蛋白 |
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| Effects of Astaxanthin on the Collagen Expression of Cardiac Fibroblasts Induced by Angiotensin Ⅱ |
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| Abstract:Objective To investigate the effect of astaxanthin on the collagen expression in cardiac fibroblasts induced by AngiotensinⅡ(AngⅡ).Methods We cultured cardiac fibroblasts(CFs) of neonatal Sprague-Dawley(SD)rats in vitro.The CFs were divided into five groups which were control group cultured without AngⅡor astaxanthin,AngⅡgroup cultured with AngⅡand astaxanthin groups cultured with AngⅡand astaxanthin 20,40,80μmol/L,respectively.After 24 hours of cultivation,the level of collagenⅠwas detected by Western bloting, the expression of the α-smooth muscle actin (α-SMA)was detected by reverse transcription polymerase chain reaction(RT-PCR),and the proliferation of CFs was measured by CCK8 kit.Results The expression of collagenⅠand α-SMA were increased induced by AngⅡ(P<0.05),and the proliferation of CFs was also enhanced by AngⅡ,but astaxanthin reversed these effects during co-treating with AngⅡ(P<0.05).Conclusion Within a range of given concentrations,astaxanthin can reduce the expression of collagenⅠand α-SMA,and inhibit the proliferation of CFs,which indicate that astaxanthin can inhibit cardiac fibrosis. |
| keywords:Astaxanthin Cardiac fibroblast Cardiac fibrosis Collagen Ⅰ |
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