| 姜黄素对肺炎链球菌感染的抗炎和抑菌作用 |
| 投稿时间：2017-05-07 修订日期：2017-05-24 点此下载全文 |
| 引用本文：余璐,李海燕,温顺航,李昌崇.姜黄素对肺炎链球菌感染的抗炎和抑菌作用[J].医学研究杂志,2018,47(2):87-91 |
| DOI：
10.11969/j.issn.1673-548X.2018.02.021 |
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| 基金项目:浙江省自然科学基金资助项目（LZ13H010001）；温州市科技局基金资助项目（Y20160243） |
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| 中文摘要:目的 研究姜黄素（Cur）及其衍生物6B、Y20对肺炎链球菌（streptococcus pneumonia，SP）最小抑菌浓度及对其致病因子NanA、NF-κB表达调节，探讨Cur、6B、Y20对SP抑菌作用及减少SP感染炎性反应的作用机制。方法 利用微量浓度稀释法联合血琼脂平板涂布法检测Cur、6B、Y20对SP的最小抑菌浓度（minimum inhibitory concentration，MIC）值；培养人支气管上皮（BEAS-2B），建立体外SP感染模型，分为空白对照组、SP感染组、Cur+SP组、6B+SP组、Y20+SP组。CCK8法测试细胞存活率，ELISA法检测NanA、TNF-α等的蛋白含量，用Western blot法检测NF-κB的表达。结果 Cur、6B、Y20在高浓度有抑菌作用；随SP感染时间的延长，药物处理组细胞存活率较SP感染组相比增高（P<0.01）；与空白对照组比较，SP感染组中NanA、NF-κB、TNF-α表达增加；与SP感染组比较，Cur、6B、Y20药物预处理组NF-κB、NanA表达降低（P<0.05），TNF-α等表达水平较模型降低（P<0.01）。结论 Cur、6B、Y20高浓度有抑菌作用，并可通过下调SP表面毒力因子NanA的表达，减轻SP的毒力作用，并通过抑制NF-κB转录激活，减少下游炎性因子表达，减轻SP引起炎症损伤。 |
| 中文关键词:姜黄素及其衍生物 肺炎链球菌 最小抑菌浓度 核转录因子-κB 神经酰胺酶A |
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| Effective of Cucrcumin on Anti-bacterial and Anti-inflammation Induced by Streptococcus Pneumoniae |
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| Abstract:Objective To study the the minimum inhibitory concentration of curcumin and curcuminoids such as 6B and Y20 on the inhibition of streptococcus pneumoniae(SP),and the regulation efficacy of curcumin and curcuminoids on NF-κB and NanA expression,in order to discuss the anti-bacteria effect and mechanism of anti-inflammatory of Cur, 6B,Y20 against streptococcus pneumoniae infection.Methods According to micro dilution method combined with blood agar plate coating method, we calculated the MIC of Cur,6B,Y20 on streptococcus pneumoniae. dThe SP infection model was established in vitro and performedby human bronchial epithelium(BEAS-2B) divided into blank control group,SP infection group and Cur+SP groups,6B+SP groups,Y20+SP groups. Cell survival rate was measured by CCK8.The protein level of NanA,TNF-α was measured by ELISA,the expression of NF-κB was measured by Western blot.Results Cur,6B,Y20 entirely inhibited the growth of SP at high concentrations. Over the time of infection,the survival rate was higher in Cur,Y20,6B treated groups compared with SP groups(P<0.01),and compared with the SP infection group, the expressions of NF-κB,NanA were decreased in the pre-treatment groups(P<0.05),also TNF-α expression reduced (P<0.01).Conclusion Cur,Y20,6B had an anti-bacteria effect at high concentrations. They could reduce the virulence of SP by effectively down-regulating the expression of NanA,decrease the inflammation injury by inhibiting NF-κB activation and the expression of inflammation cytokines. |
| keywords:curcumin and curcuminoids SP MIC NF-κB NanA |
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