葛根素对重度烧伤后心肌组织损伤和炎性反应影响的研究
投稿时间:2016-12-06  修订日期:2017-01-12  点此下载全文
引用本文:童海东,陈骅,戴如冰.葛根素对重度烧伤后心肌组织损伤和炎性反应影响的研究[J].医学研究杂志,2018,47(2):172-176
DOI: 10.11969/j.issn.1673-548X.2018.02.041
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作者单位E-mail
童海东 314000 武警浙江总队嘉兴医院烧伤整形外科  
陈骅 314000 武警浙江总队嘉兴医院烧伤整形外科  
戴如冰 314000 武警浙江总队嘉兴医院烧伤整形外科 wujingthd@163.com 
中文摘要:目的 研究葛根素(Puerarin)对大鼠重度烧伤后心肌组织损伤和炎性反应的抑制作用及机制。方法通过92℃水浴18s复制30% TBSA Ⅲ度烧伤模型大鼠;设假烧伤组、模型对照组和葛根素(50、100、200、400mg/kg)组;观察心肌组织形态结构变化,测定血清中心肌酶和肌钙蛋白T (cTnT)含量、心肌组织中抗氧化酶活性和丙二醛(MDA)含量;检测心肌细胞凋亡状况及凋亡相关蛋白表达;检测血清中炎性细胞因子含量水平。结果 与模型对照组比较,葛根素组大鼠心肌组织形态结构病理性改变明显改善,以200mg/kg组效果最为显著;葛根素(100、200、400mg/kg)组心肌酶AST、CPK、LDH含量和cTnT含量均显著降低,SOD、CAT、MPO活性显著升高且MDA含量显著降低,心肌细胞凋亡状况明显改善,凋亡指数(AI)显著降低,cleaved caspase-3、Bax蛋白表达显著下调、Bcl-2显著上调,Bcl-2/Bax显著升高,炎性细胞因子TNF-α、IL-6、IL-8含量显著降低,200mg/kg组IL-1含量显著降低、IL-10含量显著升高,差异有统计学意义(P<0.05)。结论 葛根素对重度烧伤后心肌组织损伤和炎性反应均具有一定的抑制作用,其机制可能与葛根素能够改善抗氧化酶活性、调节凋亡相关蛋白表达以及影响炎性细胞因子含量有关。
中文关键词:葛根素  重度烧伤  心肌组织  氧化应激  炎症
 
Effects of Puerarin on Myocardial Tissue Injury and Inflammatory Response in Severely Burned Rats
Abstract:Objective To investigate the effects of Puerarin on myocardial tissue injury and inflammatory response in severely burned rats and its mechanism.Methods The model rats with 30% TBSA (three degree) were made by bathing in 92℃ water for 18s; Sham burn group, model control group, puerarin 50, 100, 200, 400mg/kg treated groups were set. The histopathological changes of myocardial tissue were observed. The content of myocardial enzymes and cTnT in serum was determined. The activity of antioxidase and the content of MDA in myocardial tissue was measured. The apoptosis of cardiomyocytes was observed. The expression of apoptosis-related proteins was determined. The level of inflammatory cytokines in serum was determined.Results Compared with model control group, the histopathological changes of puerarin treated groups were significantly improved, especially the 200mg/kg group. The content of AST, CPK, LDH, cTnT in serum of Puerarin(100, 200, 400mg/kg) treated groups were significantly decreased. The activity of SOD, CAT, MPO were significantly increased and the content of MDA was significantly decreased. The apoptosis of cardiomyocytes was significantly improved, and the AI was significantly decreased.The expression of cleaved caspase-3, Bax were significantly down-regulated and the Bcl-2 was significantly up-regulated, the Bcl-2/Bax was significantly increased. The levels of TNF-α, IL-6, IL-8 in serum were significantly decreased, the level of IL-1 in serum of puerarin 200mg/kg treated group was significantly decreased and the IL-10 was significantly increased. All of the differences above were significant(P<0.05).Conclusion Puerarin has inhibitive effects on myocardial tissue injury and inflammatory responses in severely burned rats, which was perhaps related to its effects of improving antioxidant enzyme activity, altering the expression of apoptosis-related proteins and affecting the content of inflammatory cytokines.
keywords:Puerarin  Burned  Myocardial tissue  Oxidative stress  Inflammatory
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