MIF基因启动子区-173多态性与冠心病相关性研究
投稿时间:2017-04-07  修订日期:2017-07-22  点此下载全文
引用本文:钱鲁,殷日鹏.MIF基因启动子区-173多态性与冠心病相关性研究[J].医学研究杂志,2018,47(4):32-35
DOI: 10.11969/j.issn.1673-548X.2018.04.009
摘要点击次数: 897
全文下载次数: 774
作者单位E-mail
钱鲁 325000 温州医科大学附属第二医院  
殷日鹏 325000 温州医科大学附属第二医院 qianluwz@163.com 
基金项目:国家自然科学基金资助项目(81573185);浙江省自然科学基金资助项目(2014C33163);温州市科技局基金资助项目(Y20130167)
中文摘要:目的 探讨巨噬细胞移动抑制因子(MIF)基因启动子区-173微卫星多态性与冠心病的相关性。方法 根据冠脉造影结果,将347例住院患者分为对照组229例和冠心病组118例,采用酶联免疫吸附试验(ELISA)检测血清MIF浓度,应用聚合酶链反应(PCR)技术及DNA测序技术分析基因。结果 (1)173C等位基因在冠心病组中频率较高。(2)173C等位基因的血清MIF浓度较173G等位基因高,173C/C基因型的血清MIF浓度较173C/G和173G/G基因型高。结论 本研究发现MIF-173G/C基因多态性可能与冠状动脉粥样硬化性心脏病的发展有关。
中文关键词:巨噬细胞移动抑制因子  启动子  基因多态性  冠状动脉粥样硬化性心脏病
 
Macrophage Migration Inhibitory Factor Promoter Polymorphisms (-173G/C): Relationship with Coronary Atherosclerotic Disease Subjects
Abstract:Objective We analyzed the relationship of -173G/C MIF polymorphisms with soluble MIF in Coronary Atherosclerotic Disease (CAD) patients. Methods A total of 347 patients were selected, of which 229 normal-coronary and 118 Coronary artery disease subjects. Genotyping of -173G/C polymorphisms were performed by PCR and DNA sequencing. Serum MIF levels were measured using an ELISA kit. Patients were classified by coronary angiogram. Results (1)The frequency of the C genotype was higher in CAD patients than in the control. (2) Serum MIF levels was higher in 173C subjects than in 173G subjects. In addition, we found an increase in serum MIF levels in carriers of the (C/C) genotypes the -173 MIF polymorphisms. Conclusion These data suggest that MIF -173G/C polymorphism may be related to the development of CAD in a Chinese population.
keywords:Macrophage migration inhibitory factor  Promoter  Gene polymorphisms  Coronary atherosclerotic disease
查看全文  查看/发表评论  下载PDF阅读器

京公网安备 11010502037822号