| 氢干预盆腹腔手术动物模型的构建及术后血清学指标的改变 |
| 投稿时间：2017-07-05 修订日期：2017-08-18 点此下载全文 |
| 引用本文：韩玮,朱志伟,李秦剑,张新事,康志敏,曹方,胡永伟,张聪,马俊,刘琴,顾星,丁厚中.氢干预盆腹腔手术动物模型的构建及术后血清学指标的改变[J].医学研究杂志,2018,47(4):139-142,138 |
| DOI：
10.11969/j.issn.1673-548X.2018.04.034 |
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| 中文摘要:目的 构建并评价富氢环境在干预比格犬盆腹腔手术损伤中的作用。方法 选取的10只健康清洁幼年雌性比格犬，随机分为对照组与实验组，均在术中暴露并回纳回肠、空肠、回盲部、膀胱、子宫及附件，同时用电凝刀破坏回盲部、膀胱、子宫及附件；根据吸氢后血氢浓度变化、饮用水盆2000ml富氢水浓度衰减分析以及200ml腹腔富氢生理盐水灌洗液氢浓度衰减分析，构建出盆腹腔手术围术期富氢环境条件；对照组术后予以普通处理，实验组在富氢环境下饲养与护理。结果 吸入含2%氢的混合气体30min后，血氢浓度趋于稳定；新制饱和富氢水1h后，氢浓度下降为初始浓度的一半；腹腔灌注饱和富氢生理盐水后，氢浓度急剧下降，约至15min后，浓度下降一半。根据已构建的富氢环境，检测术前、术后第1天以及第3天的WBC、MDA、SOD、IL-6以及TNF-α的水平。其中，术前两组对比差异无统计学意义（P>0.05）；术后第1天和第3天实验组WBC、MDA、IL-6以及TNF-α水平均明显低于对照组（P<0.05），而实验组SOD的水平明显高于对照组（P<0.05）。结论 本研究成功构建了一个完整的富氢环境模型，利用该模型后，盆腹腔手术术后血清炎性指标及氧化应激代谢指标明显下调，提示该富氢环境能够有效控制盆腹腔手术带来的损伤、炎性反应及氧化应激。 |
| 中文关键词:氢 犬 手术 炎症 氧化应激 |
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| Building of Intervention of Hydrogen-rich Environment During the Operation and Perioperative Period of Animal Abdominal and Pelvic Surgery and the Changes of Serological Indicators |
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| Abstract:Objective To construct and evaluate the role of hydrogen-rich environment in the injury of the abdominal and pelvic surgery. Methods We selected 10 healthy young female beagles, and randomly divided them into control group and experimental group. The ileum, jejunum, ileocecal junction, bladder, uterus and accessories of each dog were all exposed, and were destroyed by the electric coagulation knife in the operation. According to results of absorbing hydrogen, drinking hydrogen-rich water, and hydrogen-rich saline lavage, we constructed the hydrogen-rich environment. The control group was treated with normal treatment and the experimental group was cared for in the hydrogen-rich environment. Results The hydrogen concentration of blood was stabilized after 30min with a mixture absorption consisting of 2% hydrogen. The concentration of hydrogen in the water was reduced to half after 1h. The concentration of hydrogen fell by half after the peritoneal lavage with hydrogen-rich saline after 15min. Based on the rich hydrogen environment, we examined the levels of WBC, MDA, SOD, IL-6 and TNF-α. There was no significant difference between the two groups before operation (P>0.05). In both the first day and the third day after surgery, the levels of WBC, MDA, IL-6 and TNF-α in the experimental group were significantly lower than those in the control group (P<0.05), while the level of SOD in the experimental group was significantly higher than that in the control group (P<0.05). Conclusion The hydrogen-rich environment we built, down-regulated serum levels of inflammatory biomarkers and oxidative stress and metabolism indicators. These results implied that this hydrogen-rich environment could effectively control the inflammation and oxidative stress of surgery injury caused by animal abdominal and pelvic operation. |
| keywords:Hydrogen Dog Surgery Inflammation Oxidative stress |
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