Toll样受体4在乙型肝炎相关性肝癌患者外周血CD14+单核细胞表面的表达及意义
投稿时间:2017-06-02  修订日期:2017-08-23  点此下载全文
引用本文:周东晓,马英杰,杨丽,陈光耀,高晓.Toll样受体4在乙型肝炎相关性肝癌患者外周血CD14+单核细胞表面的表达及意义[J].医学研究杂志,2018,47(4):143-146
DOI: 10.11969/j.issn.1673-548X.2018.04.035
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作者单位E-mail
周东晓 450003 新乡医学院附属郑州人民医院消化科、郑州消化疾病研究所  
马英杰 450003 新乡医学院附属郑州人民医院消化科、郑州消化疾病研究所 mayingjie19@sina.com 
杨丽 450003 新乡医学院附属郑州人民医院消化科、郑州消化疾病研究所  
陈光耀 450003 新乡医学院附属郑州人民医院消化科、郑州消化疾病研究所  
高晓 450003 新乡医学院附属郑州人民医院消化科、郑州消化疾病研究所  
中文摘要:目的 通过观察TLR4在乙型肝炎相关性肝癌患者外周血CD14+单核细胞表面的表达,探讨其在疾病发生、发展中的作用。方法 实验共选取慢性乙型肝炎患者25例(慢性乙型肝炎组)、乙型肝炎并肝癌23例(乙型肝炎相关性肝癌组)、健康对照16例(对照组),利用流式细胞术检测研究对象外周血CD14+单核细胞表面TLR4阳性细胞表达百分率和平均荧光强度的几何均数(GMF),并分析TLR4阳性细胞表达百分率和GMF与内毒素水平及CRP、PCT的相关性。结果 乙型肝炎相关性肝癌组TLR4阳性细胞表达百分率和GMF分别为79.56%±8.12%、54.14±12.28,较慢性乙型肝炎组(46.15%±5.35%、32.27±6.46)及正常对照组(14.89%±8.21%、15.21±6.87)显著升高,差异有统计学意义(P<0.05)。乙型肝炎相关性肝癌患者TLR4阳性细胞百分率和TLR4 GMF与内毒素水平呈正相关(P<0.05),与临床指标PCT、CRP无显著相关性(均P>0.05)。结论 TLR4在乙型肝炎相关性肝癌患者外周血CD14+单核细胞表面表达有显著升高,提示TLR4可能在乙型肝炎相关性肝癌的发生和发展中具有促进作用。
中文关键词:Toll样受体4  乙型肝炎相关性肝癌  外周血单核细胞
 
Expression and Significance of Toll-like Receptor 4 on CD14+ Peripheral Blood Monocyte Cells Surface in Patients with HBV Related Hepatocellular Carcinoma
Abstract:Objective To study the effect and possible mechanism of Toll-like receptors(TLR4) in the development and progress of HBV related hepatocellular carcinoma. Methods To-tally 64 patients were enrolled, including 25 patients with chronic hepatitis B(CHB),23 cases with HBV related hepatocellular carcinoma and 16 healthy subjects, flow cytometry analysis was used to detect the expression of CD14+ monocyte surface TLR4 positive cells, the TLR4 expression rate, the geometric mean fluorenscence (GMF) and the related clinical indicators. Results In HBV related hepatocellular carcinoma patients, the expression rate of TLR4 in the CD14+ monocytes was 79.56%±8.12%, which was significantly higher than that of CHB patients 46.15%±5.35% and control group 14.89%±8.21%, there were significant differences among these three groups (P<0.05); the GMF of TLR4 on CD14+ monocyte in the peripheral blood was 54.14±12.28 in HBV related hepatocellular carcinoma group, 32.27(6.46 in the CHB group and 15.21(6.87 in the healthy control group,the expression of TLR4 GMF in HBV related hepatocellular carcinoma group was significantly higher than that in the CHB group (P<0.05)and control group (P<0.05). It was shown that the expression of TLR4 was positive correlated with endotox level in related hepatocellular carcinoma group(P<0.05). Conclusion The expression of TLR4 was up-regulated on CD14+ peripheral blood mononuclear cells in HBV related hepatocellular carcinoma patients. The results indicate that TLR4 play an important role in the development and progress of HBV related hepatocellular carcinoma.
keywords:Toll-like receptors 4  HBV related hepatocellular carcinoma  Peripheral blood mononuclcar cells
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