STZ诱导的2型与1型糖尿病大鼠模型的对比研究
投稿时间:2018-01-16  修订日期:2018-01-16  点此下载全文
引用本文:崔雅忠,杨丹,张倩,王超,张改丽,梁晓春,吴群励.STZ诱导的2型与1型糖尿病大鼠模型的对比研究[J].医学研究杂志,2018,47(5):36-38
DOI: 10.11969/j.issn.1673-548X.2018.05.009
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作者单位E-mail
崔雅忠 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
杨丹 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
张倩 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
王超 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
张改丽 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
梁晓春 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心  
吴群励 100730 北京协和医学院/中国医学科学院北京协和医院中医科、北京协和医院转化医学中心 chinlie@163.com 
基金项目:国家自然科学基金资助项目(81001580);北京市自然科学基金资助项目(7152122)
中文摘要:目的 观察链脲佐菌素(STZ)诱导的2型与1型糖尿病大鼠模型的差异。方法 雄性SD大鼠,高脂高糖饲养6周后按35mg/kg单次腹腔注射2%STZ溶液建立2型糖尿病(T2DM)模型,普通饲料饲养6周后按60mg/kg单次腹腔注射2%STZ溶液建立1型糖尿病(T1DM)模型,设立正常对照组。检测大鼠体重、血糖、糖化血红蛋白(HbA1c)、血脂谱及氧化型低密度脂蛋白(ox-LDL)水平。结果 与正常组比较,糖尿病组大鼠的血糖、血脂和ox-LDL水平显著升高(P<0.01);T1DM组体重显著下降(P<0.01),T2DM组体重差异无统计学意义(P>0.05)。T2DM组的体重、血脂谱及ox-LDL水平显著高于T1DM组(P <0.01),血糖差异无统计学意义(P >0.05)。结论 高脂、高糖喂养基础上单次腹腔注射STZ可成功诱导2型糖尿病大鼠模型,其体重及血脂异常显著高于1型糖尿病模型,近似于人类2型糖尿病。
中文关键词:糖尿病  大鼠模型  链脲佐菌素  高脂高糖饮食
 
Comparison of Type 2 and Type 1 Diabetic Rats Induced by Streptozotocin
Abstract:Objective To observe the difference between type 2 and type 1 diabetic rats induced by streptozotocin (STZ).Methods Male SD rats, after 6 weeks' high fat and high sugar diet and then with a single intraperitoneal injection of 2%STZ solution by dose of 35mg/kg to esbabish type 2 diabetes mellitus (T2DM) model, common diet for 6 weeks to esbabish type 1 type 1 diabetes mellitus (T1DM) model by single intraperitoneal injection of 60mg/kg 2%STZ.And the normal control group is also esbabished. The body weight, blood sugar, glycated hemoglobin (HbA1c), blood lipid spectrum and oxidized low density lipoprotein (ox-LDL) level were measured.Results Compared with the normal group, the blood glucose, lipid and ox-LDL levels in the diabetic group increased significantly (P<0.01), the weight in the T1DM group decreased significantly (P<0.01), and there was no significant difference in the T2DM group (P>0.05). The body weight, blood lipid spectrum and ox-LDL level in T2DM group were significantly higher than those in T1DM group (P<0.01). There was no significant difference in blood sugar between T1DM and T2DM groups (P>0.05).Conclusion A single intraperitoneal injection of STZ in rats on the basis of high fat and high glucose diet can induce type 2 diabetes model similar to human type 2 diabetes, and their body weight and blood lipid abnormalities are significantly higher than those of type 1 diabetes.
keywords:Diabetes mellitus  Rat model  Streptozotocin  High fat and glucose diet
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