PLP2在心肌重构中的动态表达变化
投稿时间:2017-08-16  修订日期:2017-08-29  点此下载全文
引用本文:吴青青,唐其柱.PLP2在心肌重构中的动态表达变化[J].医学研究杂志,2018,47(6):25-28
DOI: 10.11969/j.issn.1673-548X.2018.06.008
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作者单位E-mail
吴青青 430060 武汉大学人民医院心内科、武汉大学心血管病研究所、心血管病湖北省重点实验室  
唐其柱 430060 武汉大学人民医院心内科、武汉大学心血管病研究所、心血管病湖北省重点实验室 qztang@whu.edu.cn 
基金项目:国家自然科学基金资助项目(81700353)
中文摘要:目的 探讨PLP2蛋白在心肌重构心脏组织和心脏各类细胞中的表达改变。方法 用冠状动脉左前降支结扎术建立小鼠心肌梗死后的心脏重构模型、异丙肾上腺素(ISO)皮下注射2周建立小鼠急性心脏损伤模型;胸主动脉结扎术(aortic banding,AB)建立小鼠心肌肥厚的模型。苯肾上腺素诱(PE)导心肌细胞肥大模型;转化生长因子(TGF)β刺激心肌成纤维细胞激活。采用RT-PCR检测上述各种心脏重构模型中PLP2转录水平的改变。结果 PLP2在小鼠心肌梗死(miocardial infarction,MI)术后2周表达明显增高(P<0.05);在ISO诱导的急性心脏损伤后2周表达增高(P<0.05);在AB术后1周表达开始增高(P<0.05),在AB术后2周达到高峰(P<0.05),持续到AB术后8周(P<0.05)。PLP2在PE诱导的心肌细胞肥大模型中表达增高;在TGFβ刺激的成纤维细胞中表达明显增高。结论 PLP2的表达在不同模型的心脏重构中均发生明显改变,且呈现动态变化,提示其可能参与心脏重构的发生、发展。
中文关键词:蛋白脂质蛋白2  心肌梗死  异丙肾上腺素  胸主动脉结扎术  心肌重构
 
Expression Alteration of PLP2 in the Process of Cardiac Remodeling
Abstract:Objective To investigate the expression of PLP2 protein in the process of cardiac remodeling. Methods Mice were subjected to left anterior descending coronary artery ligation to induce cardiac remodeling model. Mice were subjected to isoproterenol (ISO) subcutaneous injection for 2 weeks to establish acute cardiac injury model. Mice were subjected to aortic banding (AB) surgery to establish a myocardial hypertrophy model. Cardiac myocytes hypertrophy model was induced by phenylephrine (PE) stimulation. Transforming growth factor (TGF) beta was used to stimulate cardiac fibroblasts. The PLP2 transcription level was detected by RT-PCR. Results PLP2 expression was significantly increased in 2 weeks after myocardial infarction (P<0.05), as well as in acute cardiac injury induced by ISO injection (P<0.05). After 1 week of AB surgery, the PLP2 expression began to increase (P<0.05), peaked at 2 weeks post AB (P<0.05), preserved to 8 weeks after AB (P<0.05). The expression of PLP2 in PE stimulated cardiomyocytes was increased as well as TGFβ stimulated fibroblast. Conclusion The expression of PLP2 were dynamically changed significantly in different cardiac remodeling model, suggesting that it may be involved in the occurrence and development of cardiac remodeling.
keywords:Proteolipid protein 2  Cardiac remodeling  Myocardial infarction  Isoproterenol  Aortic banding
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