| 云南彝族Fetuin A基因多态性与2型糖尿病合并下肢动脉硬化的相关性研究 |
| 投稿时间：2017-06-15 修订日期：2017-07-09 点此下载全文 |
| 引用本文：刘娜,杨秋萍,张健伟.云南彝族Fetuin A基因多态性与2型糖尿病合并下肢动脉硬化的相关性研究[J].医学研究杂志,2018,47(6):45-51 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.012 |
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| 基金项目:云南省自然科学基金资助项目（2009CD166） |
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| 中文摘要:目的 研究云南彝族人群中Fetuin A基因rs4917、rs4918、rs1071592、rs2248690单核苷酸多态性（single nucleotide polymorphisms，SNPs）的基因型、等位基因频率分布特征，及其与2型糖尿病合并下肢动脉硬化的相关性。方法 采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和等位基因特异引物-聚合酶链反应（ASP-PCR）技术检测120例健康对照组、61例2型糖尿病组、67例糖尿病合并下肢动脉硬化组Fetuin A基因4个位点的SNP。结果 Fetuin A基因rs1071592（C/A）位点的C/C型基因频率及C等位基因频率在2型糖尿病合并下肢动脉粥样硬化组高于健康对照组（P<0.01）；rs4917（C/T）、rs4918（C/G）、rs2248690（A/T）在3组中其基因型及等位基因频率差异无统计学意义（P>0.05）。Logistic回归分析显示糖尿病下肢动脉粥样硬化的发生与Fetuin A基因rs1071592位点CC基因型相关。结论 在中国楚雄地区彝族中存在Fetuin A基因rs4917（C/T）、rs4918（C/G）、rs1071592（C/A）、rs2248690（A/T）多态性。在云南彝族人群Fetuin A基因rs1071592位点CC基因型可能是糖尿病下肢动脉粥样硬化的危险因素。 |
| 中文关键词:Fetuin A基因 单核苷酸多态性 2型糖尿病 动脉粥样硬化 彝族 |
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| Study on the Correlation of Fetuin A Gene Polymorphism and Lower Extremity Atherosclerosis with Type 2 Diabetes of Yi Nationality in Yunnan Province |
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| Abstract:Objective To investigate the Allele and genotype frequency distribution of four single nucleotide polymorphisms(SNPs) sites(rs4917,rs4918,rs1071592,rs2248690) in Fetuin A gene with type 2 diabetes and type 2 diabetes complicated by atherosclerosis of lower limb arteries(ALLA) in Yi Nationality of Chuxiong, and evaluated for association of Fetuin A polymorphisms with ALLA in type 2 diabetes. Methods Four SNPs in Fetuin A were genotyped in One hundred twenty normal controls, sixty one T2DM cases and sixty seven ALLA in type 2 diabetes by polymerase chain reaction-restricted fragment length polymorphism(PCR-RFLP) and allelic specific primer-polymerase chain reaction(ASP-PCR). Results The CC genotype and Allele C of rs1071592 in normal controls was lower than those which ALLA in type 2 diabetes(P<0.01),and there were no significant differences in frequency of genotype and allele distributions in the polymorphism analysis of rs4917(C/T),rs4918(C/G),rs2248690(A/T) in these three groups(P>0.05).Logistic regression analysis showed:the CC genotype of rs1071592 in Fetuin A were nominally associated with atherosclerosis of lower limb arteries of T2DM. Conclusion There existed polymorphism of 4917(C/T),rs4918(C/G),rs2248690(A/T),rs1071592(C/A) of Fetuin A in Yi nationality of chuxiong.The CC genotype of rs1071592 might be genetic risk factors of type 2 diabetes complicated by ALLA. |
| keywords:Fetuin A gene Single nucleotide polymorphism(SNP) Type 2 diabetes mellitus Atherosclerosis Yi Nationality |
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