| 神经干细胞脑出血大鼠脑内移植对T淋巴细胞亚群的影响 |
| 投稿时间：2017-09-21 修订日期：2017-09-23 点此下载全文 |
| 引用本文：高鲁,叶雷,李仲颖,单明,程宏伟.神经干细胞脑出血大鼠脑内移植对T淋巴细胞亚群的影响[J].医学研究杂志,2018,47(6):57-63 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.014 |
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| 基金项目:安徽省自然科学基金资助项目（1708085MH211） |
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| 中文摘要:目的 探讨神经干细胞（neural stem cells，NSCs）移植入脑出血大鼠模型脑内后，观察脑组织内和血液中T淋巴细胞亚群的变化。方法 选择成年雄性SD大鼠作为模型动物，采用Ⅳ型胶原酶诱导法建立脑出血模型，脑出血后3h移植神经干细胞，3天进行各项指标检测。应用流式细胞仪分析脑组织与血液中的T淋巴细胞亚群变化。应用酶联免疫吸附试验检测脑组织与血液中细胞因子的变化。通过组织免疫荧光了解T淋巴细胞与NSCs在脑出血大鼠脑内的分布情况。结果 在血液中，与对照组相比较，NSCs移植组能够增加调节性T细胞（regulatory T cells，Treg）与CD4+T细胞表达，减少γδT与CD8+T细胞表达。在脑组织中，NSCs移植组能够增加Treg细胞表达，减少γδT、CD4+T和CD8+T细胞表达。与对照组比较，NSCs移植组增加血液与脑组织中IL-4（interleukin-4）、IL-10（interleukin-10）和TGF-β（transforming growth factor-β）的表达，并且减少IL-6（interleukin-6）与IFN-γ（interferon-γ）的表达。结论 脑出血早期NSCs脑内移植促进保护性的T细胞亚群和抑制破坏性的T细胞亚群表达，提示移植的NSCs可能通过发挥免疫调节作用起到神经保护作用。 |
| 中文关键词:脑出血 神经干细胞移植 T淋巴细胞亚群 免疫调节作用 |
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| Effects of Neural Stem Cells Intracerebral Transplantion on T Lymphocyte Subpopulations in Intracerebral Hemorrhage Rats |
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| Abstract:Objective To discuss the role of neural stem cells(NSCs) intracerebral transplantation in the modulation of T lymphocyte subpopulations in the brain and blood after intracerebral hemorrhage in rats. Methods NSCs were injected intracerebrally at 3 hours after collagenase induced ICH in sprague dawley rat models. All experimental results were tested at 3 days after ICH. Flow cytometry was used to quantify T lymphocyte subpopulations in the brain and blood 3 days post-ICH. Cytokine enzyme-linked immunosorbent assay(ELASA) investigated changes in the levels of inflammatory mediators. Immunohistochemistry detect distribution of injected NSCs and invaded T lymphocyte in the brain. Results In the blood, Treg cells and CD4+T cells increased but γδT and CD8+T cells decreased in the NSCs-ic-3h group compared to the vehicle-injected control animals. NSCs-ic-3h group detected Treg cells infiltration increasion and γδT, CD4+T and CD8+T cells infiltration decreasion in the brain after ICH. We also observed that expression of anti-inflammatory cytokines, IL-4, IL-10 and TGF-β increased while expression of pro-inflammatory cytokines, IL-6, and IFN-γ decreased in the brain and blood after NSCs transplantation. Conclusion Our data suggest that early intracerebral NSCs injection after ICH protect brain injury mainly by promoting protective T lymphocyte expression and inhibiting detrimental T lymphocyte expression. |
| keywords:ICH NSCs transplantation T lymphocyte subpopulations Immunomodulatory effects |
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