| siRNA沉默HLX基因对AML细胞生长的影响 |
| 投稿时间：2017-08-09 修订日期：2017-09-22 点此下载全文 |
| 引用本文：朱夏吟,陈伟卫,张丹琼,罗文达.siRNA沉默HLX基因对AML细胞生长的影响[J].医学研究杂志,2018,47(6):77-81 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.018 |
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| 基金项目:浙江省医药卫生科技计划项目（2014KYB309、2017KY709） |
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| 中文摘要:目的 筛选高表达H2.0样同源盒基因（H2.0-like homeobox gene，HLX）的白血病细胞株，沉默HLX后观察急性髓系白血病（acute myelogenous leukemia，AML）细胞株的生长及分化影响，为HLX在AML中的深入研究提供理论基础。方法 实时荧光定量PCR（quantitative real-time polymerase chain reaction，qRT-PCR）筛选高表达HLX基因的白血病细胞株，并检测p21活化激酶1（p21-activated kinase 1，PAK1）的基因表达；设计siRNA-HLX沉默HLX基因表达最高的AML细胞株中的该基因，运用相差显微镜观察细胞生长情况，MTS/PMS比色分析法检测增殖抑制率。结果 AML细胞株中HLX基因的表达水平显著较高（P< 0.01），干扰HLX基因后，可见AML细胞株生长速度减慢，增殖被抑制，并且PAK1mRNA表达下调（P< 0.01）。结论 HLX基因可抑制AML细胞株的生长、增殖及分化。 |
| 中文关键词:HLX基因 siRNA AML细胞株 增殖 |
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| Effect of Growth of Silencing HLX Gene in the AML Cells by siRNA in vitro |
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| Abstract:Objective To investigate the expression of HLX gene in leukemia cell lines, and to analyze the growth of cells and the expression of PAK1 gene by inhibiting HLX expression with RNA interference technique. Methods According to HLX gene coding sequence, siRNA-HLX was transfected into AML/M3 subtype NB4 cells to inhibition the HLX gene, and the expression of HLX gene and PAK1 gene was detected by qRT-PCR, and the cell growth was observed by fluorescence phase contrast microscope, MTS/PMS was used to detect proliferation inhibition rate. Results The expression of HLX gene in AML cell lines was significantly higher than that in control group (P<0.01). When HLX gene was down-regulated, the expression of PAK1 mRNA was also down-regulated (P<0.01), and AML cells grow slowly and its proliferation is inhibited. Conclusion HLX gene can inhibit the growth,proliferation and differentiation of AML cell lines. |
| keywords:HLX siRNA AML cell lines Proliferation |
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