| 幽门螺杆菌毒力基因与克拉霉素耐药基因突变相关性 |
| 投稿时间：2018-04-14 修订日期：2018-04-24 点此下载全文 |
| 引用本文：章月桃,陈伟燕,朱临江,刘云惠,孟飞.幽门螺杆菌毒力基因与克拉霉素耐药基因突变相关性[J].医学研究杂志,2018,47(6):82-84,143 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.019 |
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| 基金项目:浙江省医药卫生科技计划项目（2018KY908）；浙江省科技厅公益技术应用研究项目（2016C33232、2017C33192） |
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| 中文摘要:目的 分析浙江省台州地区幽门螺杆菌（helicobacter pylori，Hp）cagA和VacA为主的毒力基因型分布，探讨其与克拉霉素耐药基因突变类型的关系。方法 招募存在上消化道症状患者，并行胃镜检查活检患者胃黏膜组织。通过Hp分离培养方法，培养出216株Hp。采用聚合酶链式反应（polymerase chain reaction，PCR）检测并分析cagA和VacA的分型情况。采用PCR扩增和Sanger测序技术检测克拉霉素耐药基因（23S rRNA）2142和2143位碱基点突变情况。结果 CagA基因的阳性检出率为99.54%（215/216），其中CagA-D为95.83%（207/216）、CagA-C为3.70%（8/216）。VacA基因阳性检出率为97.22%（210/216），s1、s2、m1、m2亚型分别为97.22%（210/216）、0、35.19%（76/216）、60.65%（131/216）。本研究发现，浙江省台州地区毒力基因以CagA-D和VacAs1 m2型为优势基因型。此外，23S rRNA基因2142和2143位点均未突变的有93株。突变的有123株，其中A2142G位点突变4株、A2143G位点突变119株。毒力基因型与23S rRNA基因2142和2143位点突变类型无相关性（P>0.05）。结论 台州地区为HP高毒力地区，且以CagA-D和VacAs1 m2型为主，其分布与克拉霉素耐药基因突变无密切相关性。 |
| 中文关键词:幽门螺杆菌 毒力基因 克拉霉素耐药基因 |
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| Relationship between Virulence Genes of Helicobacter Pylori and Gene Mutation Associated with Clarithromycin Resistance |
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| Abstract:Objective To analyze the distributions of virulence genes cagA and VacA from helicobacter pylori (Hp) and to study the relationship between virulence genotype and gene mutation associated with clarithromycin resistance. Methods A total of 216 Hp were isolated from gastric mucosal tissue in patients with upper gastrointestinal symptoms. The cag A and VacA genotypes were detected and analyzed by polymerase chain reaction (PCR). The mutations at positions 2142 and 2143 of clarithromycin resistance gene (23S rRNA) were detected by PCR and Sanger sequencing. Results The positive rate of CagA gene was 99.54% (215/216), of which CagA-D was 95.83% (207/216) and CagA-C was 3.70% (8/216). The positive rate of VacA gene was 97.22% (210/216), and the s1, s2, m1, and m2 subtypes were 97.22% (210/216), 0, 35.19% (76/216) and 60.65% (131/216), respectively. In this study, CagA-D and VacAs1m2 were dominant genotypes in Taizhou region. Additionally, there were 93 Hp strians which were not mutated in 2142 and 2143 gene loci of 23S rRNA. There were 123 Hp mutants, of which 4 strains were A2142G mutations and 119 strains were A2143G mutations. There were no relationship between virulence genotype and 2142 and 2143 gene loci of 23S rRNA mutation types (P>0.05). Conclusion Taizhou is a region with high Hp toxicity and is mainly in CagA-D and VacAs1m2. The distribution of the Hp toxicity is not closely related to the clarithromycin-resistance gene mutation. |
| keywords:Helicobacter pylori Virulence genes Clarithromycin resistance gene |
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